Journal Article Epublish 3 citations
PubMed: 39458579 DOI: 10.3390/pharmaceutics16101247
Phytosynthesis of Silver Nanoparticles Using (Lam.) A.H. Gentry (Bignoniaceae) Leaf Extract: Characterization and Their Biological Activities.
Pharmaceutics 16(10)
Authors: Johana Zuñiga-Miranda, Saskya E Carrera-Pacheco, Rebeca Gonzalez-Pastor, Arianna Mayorga-Ramos, Cristina Rodríguez-Pólit, Jorge Heredia-Moya, Karla Vizuete, Alexis Debut, Carlos Barba-Ostria, Elena Coyago-Cruz, Linda P Guamán
Affiliations
- Centro de Investigación Biomédica CENBIO, Facultad de Ciencias de la Salud Eugenio Espejo, Universidad UTE, Quito 170527, Ecuador.
- Centro de Nanociencia y Nanotecnología, Universidad de Las Fuerzas Armadas ESPE, Sangolquí 171103, Ecuador.
- Departamento de Ciencias de la Vida y Agricultura, Universidad de las Fuerzas Armadas ESPE, Sangolquí 171103, Ecuador.
- Escuela de Medicina, Colegio de Ciencias de la Salud Quito, Universidad San Francisco de Quito (USFQ), Quito 170901, Ecuador.
- Instituto de Microbiología, Universidad San Francisco de Quito (USFQ), Quito 170901, Ecuador.
- Carrera de Ingeniería en Biotecnología de los Recursos Naturales, Universidad Politécnica Salesiana, Quito 170143, Ecuador.
Abstract
. is a native plant renowned for its medicinal properties in traditional healing in the Amazon Region. This plant is rich in polyphenols, flavonoids, anthocyanins, phenolic acids, tannins, ketones, triterpenes, as well as other bioactive compounds. . This study aims to develop an innovative, eco-friendly method for synthesizing silver nanoparticles using an aqueous extract of (Ma-AgNPs), enhancing the biological activities of AgNPs by leveraging the therapeutic potential of the plant's bioactive compounds. . Silver nanoparticles were synthesized using the aqueous extract of . The biological activities of Ma-AgNPs were assessed, including antibacterial, anti-inflammatory, antioxidant, antitumor, and anti-biofilm effects, along with evaluating their hemolytic activity. . Quantitative analysis revealed that Ma-AgNPs exhibit potent antibacterial activity against multidrug and non-multidrug-resistant bacteria, with MIC values ranging from 1.3 to 10.0 µg/mL. The Ma-AgNPs significantly reduced NO production by 86.9% at 4 µg/mL, indicating strong anti-inflammatory effects. They demonstrated robust antioxidant activity with an IC50 of 5.54 ± 1.48 µg/mL and minimal hemolytic activity, with no hemolysis observed up to 20 µg/mL and only 4.5% at 40 µg/mL. Their antitumor properties were notable, with IC50 values between 2.9 and 5.4 µg/mL across various cell lines, and they achieved over 50% biofilm inhibition at concentrations of 30-40 µg/mL. . These findings underscore the potential of Ma-AgNPs for biomedical applications, particularly in developing new antimicrobial agents and bioactive coatings with reduced toxicity. This research highlights a sustainable approach that not only preserves but also amplifies the inherent biological activities of plant extracts, paving the way for innovative therapeutic solutions.
MeSH
Nanoparticles, Biofilms, Antioxidants, Therapeutics, Bacteria