Journal Article Preprint Epublish 16 citations
PubMed: 39464004 DOI: 10.1101/2024.10.14.618224
Diversity of ancestral brainstem noradrenergic neurons across species and multiple biological factors.
bioRxiv
Authors: Michael A Kelberman, Ellen Rodberg, Ehsan Arabzadeh, Chloe J Bair-Marshall, Craig W Berridge, Esther Berrocoso, Vincent Breton-Provencher, Daniel J Chandler, Alicia Che, Oscar Davy, David M Devilbiss, Anthony M Downs, Gabrielle Drummond, Roman Dvorkin, Zeinab Fazlali, Robert C Froemke, Erin Glennon, Joshua I Gold, Hiroki Ito, Xiaolong Jiang, Joshua P Johansen, Alfred P Kaye, Jenny R Kim, Chao-Cheng Kuo, Rong-Jian Liu, Yang Liu, Meritxell Llorca-Torralba, Jordan G McCall, Zoe A McElligott, Andrew M McKinney, Cristina Miguelez, Ming-Yuan Min, Alexandra C Nowlan, Mohsen Omrani, Gina R Poe, Anthony Edward Pickering, Yadollah Ranjbar-Slamloo, Jone Razquin, Charles Rodenkirch, Anna C Sales, Rath Satyasambit, Stephen D Shea, Mriganka Sur, John Arthur Tkaczynski, Sonia Torres-Sanchez, Akira Uematsu, Chayla R Vazquez, Amelien Vreven, Qi Wang, Barry D Waterhouse … and 7 other authors
Affiliations
- Department of Molecular, Cellular and Developmental Biology, University of Colorado Boulder, Boulder, CO, USA.
- Department of Human Genetics, Emory University, Atlanta, GA, USA.
- Department of Biology, University of Massachusetts Amherst, Amherst, MA, USA.
- Neuroscience and Behavior Program, University of Massachusetts Amherst, Amherst, MA, USA.
- Eccles Institute of Neuroscience, John Curtin School of Medical Research, The Australian National University, Canberra, AUS.
- Neuroscience Institute, NYU Langone Medical Center, New York University, New York, New York, USA.
- Department of Psychology, University of Wisconsin-Madison, Madison, WI, USA.
- Neuropsychopharmacology and Psychobiology Research Group, Department of Neuroscience, School of Medicine, Biomedical Research and Innovation Institute of Cádiz (INiBICA), University of Cadiz, Cadiz, Spain.
- Centro de Investigación Biomédica en Red de Salud Mental (CIBERSAM), Instituto de Salud Carlos III, Madrid, Spain.
- Department of Psychiatry and Neuroscience, Université Laval, Québec, QC, Canada.
- Department of Neuroscience, Rowan-Virtua SOM, Stratford, NJ, USA.
- Department of Psychiatry, Yale University School of Medicine, New Haven, CT, USA.
- Wu Tsai Institute, Yale University, New Haven, CT, USA.
- School of Physiology, Pharmacology and Neuroscience, University of Bristol, Bristol, United Kingdom.
- Bowles Center for Alcohol Studies, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
- Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
- Department of Brain and Cognitive Sciences, Picower Institute for Learning and Memory, Massachusetts Institute of Technology, Cambridge, MA, USA.
- Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA.
- School of Cognitive Sciences, Institute for Research in Fundamental Sciences (IPM), Tehran, Iran.
- Department of Psychiatry, Columbia University, New York, NY, USA.
- New York State Psychiatric Institute, New York, NY, USA.
- Department of Otolaryngology, NYU Grossman School of Medicine, New York, NY, USA.
- Department of Neurology, Weill Cornell Medicine, New York.
- Department of Neuroscience, University of Pennsylvania, Philadelphia, PA, USA.
- Department of Urology, Yokohama City University Graduate School of Medicine, Yokohama, Japan.
- Department of Neuroscience, Baylor College of Medicine Neurological Research Institute at Texas Children's Hospital, 1250, Houston, TX, USA.
- Department of Ophthalmology, Baylor College of Medicine Neurological Research Institute at Texas Children's Hospital, 1250, Houston, TX, USA.
- RIKEN Center for Brain Science, Wako-shi Saitama, Japan.
- Clinical Neurosciences Division, VA National Center for PTSD, West Haven, CT, USA.
- Department of Anesthesiology, Washington University in St. Louis, St. Louis, MO, USA.
- Department of Life Science, College of Life Science, National Taiwan University, Taipei, Taiwan.
- Department of Biomedical Engineering, Columbia University, New York, NY, USA.
- Department of Psychiatry, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
- Department of Pharmacology, Faculty of Medicine and Nursing, University of the Basque Country (UPV/EHU), Leioa, Bizkaia, Spain.
- Department of Psychiatry, Queen's University, Kingston, ON, Canada.
- Integrative Biology and Physiology, UCLA, Los Angeles, CA, USA.
- Department of Computer Science, Tokyo Institute of Technology, Midori, Yokohama, Japan.
- Human Informatics and Information Research Institute, National Institute of Advanced Industrial Science and Technology, Japan.
- Helsinki Institute of Life Science (HiLIFE), University of Helsinki, Helsinki, Finland.
- Neuroscience Center, University of Helsinki, Helsinki, Finland.
- Faculty of Pharmacy, University of Helsinki, Helsinki, Finland.
Abstract
The brainstem region, locus coeruleus (LC), has been remarkably conserved across vertebrates. Evolution has woven the LC into wide-ranging neural circuits that influence functions as broad as autonomic systems, the stress response, nociception, sleep, and high-level cognition among others. Given this conservation, there is a strong possibility that LC activity is inherently similar across species, and furthermore that age, sex, and brain state influence LC activity similarly across species. The degree to which LC activity is homogenous across these factors, however, has never been assessed due to the small sample size of individual studies. Here, we pool data from 20 laboratories (1,855 neurons) and show diversity across both intrinsic and extrinsic factors such as species, age, sex and brain state. We use a negative binomial regression model to compare activity from male monkeys, and rats and mice of both sexes that were recorded across brain states from brain slices or under different anesthetics or during wakefulness . LC activity differed due to complex interactions of species, sex, and brain state. The LC became more active during aging, independent of sex. Finally, in contrast to the foundational principle that all species express two distinct LC firing modes ("tonic" or "phasic"), we discovered great diversity within spontaneous LC firing patterns. Different factors were associated with higher incidence of some firing modes. We conclude that the activity of the evolutionarily-ancient LC is not conserved. Inherent differences due to age and species-sex-brain state interactions have implications for understanding the role of LC in species-specific naturalistic behavior, as well as in psychiatric disorders, cardiovascular disease, immunology, and metabolic disorders.
MeSH
Neurons, Brain, Brain Stem, Sex, Biological Factors