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Journal Article Epublish 1 citation

PubMed: 39493006 DOI: 10.1016/j.ijpx.2024.100291

Multifunctional poloxamer-based thermo-responsive hydrogel loaded with human lactoferricin niosomes: study on anti-bacterial activity, accelerate wound healing, and anti-inflammation.

Int J Pharm X 8:100291

Authors: Sirikwan Sangboonruang, Natthawat Semakul, Kiattikhun Manokruang, Nuttawut Khammata, Kanyaluck Jantakee, Katanchalee Mai-Ngam, Satrawut Charoenla, Phadungkiat Khamnoi, Kanokwan Saengsawang, Usanee Wattananandkul, Sorasak Intorasoot, Khajornsak Tragoolpua

Affiliations

  • Division of Clinical Microbiology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.
  • Office of Research Administration, Chiang Mai University, Chiang Mai 50200, Thailand.
  • Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
  • Center of Excellence in Materials Science and Technology, Chiang Mai University, Chiang Mai 50200, Thailand.
  • Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
  • National Metal and Materials Technology Center, National Science and Technology Development Agency, Pathumthani 12120, Thailand.
  • Diagnostic Laboratory, Maharaj Nakorn Chiang Mai Hospital, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
  • Department of Medical Technology, Lampang Hospital, Lampang 52000, Thailand.

Abstract

Chronic wound infections are attributed to delayed tissue repair, which remains a major clinical challenge in long-term health care. Particularly, infections with antibiotic resistance have more serious effects on health, often resulting in unsuccessful treatments. Thus, antimicrobial peptide (AMP)-based therapy holds promise as a potential therapeutic approach to overcoming drug resistance. Conventional wound dressing is a passive strategy for wound care that is not capable of eradicating pathogens and promoting tissue repair. In this study, we aim to construct an advanced wound dressing; a thermo-responsive hydrogel incorporated with lactoferricin (Lfcin) niosome (Lfcin-Nio/hydrogel) for bacterial pathogen treatment. The Lfcin-loaded niosome (Lfcin-Nio) has a particle size of 396.91 ± 20.96 nm, 0.38 ± 0.01 of PdI, -10.5 ± 0.3 mV of ζ potential, and 72.30 ± 7.05 % Lfcin entrapment efficiency. Lfcin-Nio exhibited broad antibacterial activity on both drug-susceptible and drug-resistant strains, and also on bacteria residing in the biofilm matrix. The Lfcin-Nio/hydrogel was fabricated from 0.5 % / poloxamer 188-20 % w/v poloxamer 407, and supplemented with Lfcin-Nio and epidermal growth factor (EGF). The physical properties of Lfcin-Nio/hydrogels showed elasticity, swelling ability, and strong injectability with responsiveness to 33-37 °C temperatures. The biological properties of Lfcin-Nio/hydrogels exhibited a bactericidal effect against drug-resistant strains of and and showed less toxicity to the human skin fibroblast. It also promoted the healing of scratches by 55 % within 6 h, compared to the wound closure rate of 20 % in the cell control. The inflammatory response of the Lfcin-Nio/hydrogel-treated cells was reduced suppression of and mRNA expressions. From this study, Lfcin-Nio/hydrogels can be suggested as a modern wound dressing that possesses multifunctional and beneficial properties for the management of chronic wound infections.

MeSH

Cells, Hydrogel, Polyethylene Glycol Dimethacrylate, Hydrogels, Pharmaceutical Preparations, Poloxamer, Therapeutics, Tissues, Bacteria, Wound Infection, Infections

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