Journal Article Epublish
PubMed: 39555232 DOI: 10.1093/toxres/tfae187
Stress survival and longevity of Caenorhabditis elegans lacking NCS-1.
Toxicol Res (Camb) 13(6):tfae187
Authors: Luiz Alexandre Viana Magno, Sofia Helena Dias Borges Pinto, Ailla Pacheco, Daniela Valadão Freitas Rosa, Priscila Gubert, Marco Aurélio Romano-Silva
Affiliations
- Programa de Pós-Graduação em Ciências da Saúde (PPGCS), Faculdade Ciências Médicas de Minas Gerais (FCMMG), Alameda Ezequiel Dias, N° 275, Centro, 30130-110 Belo Horizonte, Minas Gerais, Brazil.
- INCT em Neurotecnologia Responsável (INCT-NeurotecR), Avenida Alfredo Balena N° 190, Santa Efigênia, 30130-100, Belo Horizonte, Minas Gerais, Brazil.
- Faculdade de Medicina, Universidade Federal de Minas Gerais (UFMG), Avenida Alfredo Balena N° 190, Santa Efigênia, 30130-100, Belo Horizonte, Minas Gerais, Brazil.
- Universidade Federal de Pernambuco (UFPE), Avenida Prof. Moraes Rego, Cidade Universitária, 50670-901, Recife, Pernambuco, Brazil.
Abstract
Although dysfunctional Ca 2+ signaling can trigger biochemical reactions that lead to cell death, the role of calcium-binding proteins (CBPs) in this process is still a topic of debate. Neuronal calcium sensor 1 (NCS-1) is a CBP that is highly conserved and has been shown to increase cell survival against various types of injuries. As such, we hypothesized that NCS-1 could also be a stress-responsive protein with potential effects on survival and longevity. To explore this possibility, we conducted experiments to examine how Caenorhabditis elegans ncs-1 mutant nematodes fared under three different stress conditions: hyperosmotic, thermal, and chemical oxidant challenges. Our results showed that while the lack of NCS-1 had no effect on survival responses to hyperosmotic and thermal stresses, ncs-1 worms demonstrated remarkable resistance to the oxidant paraquat in a dose-dependent manner. Based on these findings, we conclude that C. elegans may employ adaptive mechanisms in the absence of NCS-1 to survive specific oxidative stress stimuli.
MeSH
Cells, Stress, Physiological, Oxidants, Longevity, Calcium