Journal Article Epublish
PubMed: 39590717 DOI: 10.3390/jof10110798
Extracellular Biosynthesis, Characterization and Antimicrobial Activity of Silver Nanoparticles Synthesized by Filamentous Fungi.
J Fungi (Basel) 10(11)
Authors: Iuliana Răut, Mariana Constantin, Raluca Șuică-Bunghez, Cristina Firincă, Elvira Alexandrescu, Ioana Cătălina Gîfu, Mihaela Doni, Lucian-Gabriel Zamfir, Ana-Maria Gurban, Luiza Jecu
Affiliations
- Biotechnology and Bioanalysis Department, National Institute for Research & Development in Chemistry and Petrochemistry-ICECHIM, 202 Independentei Spl. 060021 Bucharest, Romania.
- Faculty of Pharmacy, Titu Maiorescu University, 16 Bd. Gh. Sincai, 040441 Bucharest, Romania.
- Faculty of Biology, University of Bucharest, Splaiul Independentei 91-95, 050095 Bucharest, Romania.
Abstract
The green synthesis of metal nanoparticles has received substantial attention due to their applications in various domains. The aim of the study was to obtain silver nanoparticles (AgNPs) by green synthesis with filamentous fungi, such as , , and . Fungal species were grown on nutrient media and aqueous mycelium extracts were used to reduce Ag to Ag (0). The silver nanoparticles were analyzed by various techniques, such as UV-Visible spectroscopy (UV-Vis), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), dynamic light scattering (DLS), and Zeta potential. The formation of silver nanoparticles was confirmed by UV-Vis spectroscopy and the color change of the mixture containing metal precursor and aqueous mycelium extract. FTIR displayed different functional groups as capping and reducing agents for the biosynthesis of AgNPs. SEM and TEM provided information on the particles' morphology. DLS diagrams indicated mean particle diameters in the 124-168 nm region. All biosynthesized AgNPs had negative zeta values, which is a sign of good stability. Silver nanoparticles were evaluated for antimicrobial activity, and the most active were those synthesized with metabolites from , leading to 93.75% inhibition of , 67.20% of , and 69.56% of . With the highest microbial inhibition percentage and a very good Poly Dispersion Index (Pd I), was selected as an environmentally friendly source of silver nanoparticles that could be used as a potential antimicrobial agent.
MeSH
Nanoparticles, Microscopy, Electron, Microscopy, Fungi