Journal Article Epublish
PubMed: 39590724 DOI: 10.3390/jof10110805
Swiftly Manipulates the Transcriptional Regulation in During the Early Infection Stage.
J Fungi (Basel) 10(11)
Authors: Siyu Zhang, Xinyou Wang, Wei Zeng, Leijian Zhong, Xiaoyong Yuan, Zhigang Ouyang, Ruimin Li
Affiliations
- College of Life Sciences, Gannan Normal University, Ganzhou 341000, China.
- College of Life Sciences, Gannan Normal University, Ganzhou 341000, China. National Navel Orange Engineering Research Center, Ganzhou 341000, China. Jiangxi Provincial Key Laboratory of Pest and Disease Control of Featured Horticultural Plants, Ganzhou 341000, China.
- National Navel Orange Engineering Research Center, Ganzhou 341000, China.
- Jiangxi Provincial Key Laboratory of Pest and Disease Control of Featured Horticultural Plants, Ganzhou 341000, China.
Abstract
spp. represent an economically important fruit tree crop worldwide. However, molecular mechanisms underlying the interaction between citrus and the remain largely unexplored. In this study, we analyzed the physiological and transcriptomic changes in at different stages of incubation with . The results indicated that infection rapidly triggered necrosis in the epicarp of fruits, decreased the total flavonoid contents, and suppressed the activity of catalase, peroxidase, and superoxide dismutase enzymes. Upon inoculation with , there were 4600 differentially expressed genes (DEGs) with 1754 down-regulated and 2846 up-regulated after six hours, while there were only 580 DEGs with 185 down-regulated and 395 up-regulated between six and twelve-hours post-inoculation. Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes enrichment analysis indicated that the DEGs, which exhibited consistent up-regulation, were associated with metabolic processes and stress responses. Through Weighted Gene Co-Expression Network Analysis, 11 key genes have been identified that could potentially play a role in the transcriptional regulation of this process, including the transcription factor bHLH189. Furthermore, the infection of had a notable effect on both the flavonoid metabolism and the metabolic pathways related to reactive oxygen species. Our findings help to understand the interaction between citrus and and unveil how new insights into how circumvents citrus defense mechanisms.
MeSH
Genes, Fruit, Flavonoids, Infections