System Maintenance Notice

Please note that maintenance work is scheduled between 4 AM and 5 AM - CET. During this time, you may experience reduced performance. Thanks for your patience.

Publications

Journal Article Epublish 2 citations

PubMed: 39611007 DOI: 10.2147/IJN.S479937

Enhanced Efficacy of Some Antibiotics in the Presence of Silver Nanoparticles Against Clinical Isolate of Recovered from Cystic Fibrosis Patients.

Int J Nanomedicine 19:12461-12481

Authors: Hafez Al-Momani, Hadeel Albalawi, Dua'a Al Balawi, Khaled M Khleifat, Iman Aolymat, Saja Hamed, Borhan Aldeen Albiss, Ashraf I Khasawneh, Ola Ebbeni, Ayman Alsheikh, AbdelRahman M Zueter, Jeffrey Peter Pearson, Christopher Ward

Affiliations

  • Department of Microbiology, Pathology and Forensic Medicine, Faculty of Medicine, The Hashemite University, Zarqa, 13133, Jordan.
  • Faculty of Applied Medical Sciences, The Hashemite University, Zarqa, 13133, Jordan.
  • Biology Department, College of Science, Mutah University, Mutah, Karak, 61710, Jordan.
  • Department of Anatomy, Physiology and Biochemistry, Faculty of Medicine, The Hashemite University, Zarqa, 13133, Jordan.
  • Department of Pharmaceutics & Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, The Hashemite University, Zarqa, 13133, Jordan.
  • Nanotechnology Institute, Jordan University of Science & Technology, Irbid, 22110, Jordan.
  • Department of Pharmacology and Public Health, Faculty of Medicine, The Hashemite University, Zarqa, 13133, Jordan.
  • Department of Medical Laboratory Sciences, Faculty of Allied Medical Sciences, Zarqa University, Zarqa, 13110, Jordan.
  • Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, The Hashemite University, Zarqa, 13133, Jordan.
  • Biosciences Institute, Newcastle University Medical School, Newcastle Upon Tyne, UK.
  • Translational and Clinical Research Institute, Newcastle University Medical School, Newcastle Upon Tyne, NE2 4HH UK.

Abstract

Introduction

Given the increasing frequency of drug-resistant bacteria and the limited progress in developing new antibiotics, it is necessary to explore new methods of combating microbial infections. Nanoparticles, particularly silver nanoparticles (Ag-NPs), have shown exceptional antibacterial characteristics; however, elevated concentrations of Ag-NPs can produce noticeable levels of toxicity in mammalian cells.

Aim

This study examined the potential synergistic effect of combining a low dosage of Ag-NPs and anti-pseudomonas drugs against (ATCC strain) and eleven clinical isolates from cystic fibrosis patients.

Methods

The Ag-NPs were chemically produced by utilizing a seed extract from and characterized via ultraviolet-visible spectroscopy and scanning electron microscopy. The broth microdilution technique was utilized to investigate the minimum inhibitory concentration (MIC) of Ag-NPs and eight antibiotics (Piperacillin, Ciprofloxacin, Levofloxacin, Meropenem, Amikacin, Ceftazidime, Gentamicin, Aztreonam). The fractional inhibitory concentration index (FICI) was determined via the checkerboard method to evaluate the synergistic effects of Ag-NPs and various antibiotics.

Results

The biosynthesized Ag-NPs were uniformly spherical and measured around 15 nm in size. When combined with antibiotics, Ag-NP produced statistically significant reductions in the amount of antibiotics required to completely prevent growth for all strains. The findings revealed that the MIC of Ag-NPs was 15 ug/mL for all strains which decreased substantially when administered with antibiotics at a dose of 1.875-7.5 ug/mL. The majority of Ag-NP and antibiotic combinations exhibited a synergistic or partially synergistic impact. This was particularly noticeable in combinations containing Meropenem, Ciprofloxacin, and Aztreonam (in which the FIC index was less than or equal to 0.5).

Conclusion

The findings revealed that combining Ag-NPs with antibiotics was more effective than using Ag-NPs or antibiotics in isolation and that combinations of Ag-NPs and antimicrobial agents displayed synergistic activity against the majority of strains assessed.

MeSH

Nanomedicine, Fibrosis, Nanoparticles, Pharmaceutical Preparations, Biofilms, Cystic Fibrosis, Bacteria

Cited by