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Journal Article Ppublish 4 citations

PubMed: 39638121 DOI: 10.1016/j.tjnut.2024.11.005

The Impact of Sodium Selenite and Seleno-L-Methionine on Stress Erythropoiesis in a Murine Model of Hemolytic Anemia.

J Nutr 155(2):540-548

Authors: Hangdi Gong, Yuting Bai, Dane Rahoi, Robert F Paulson, K Sandeep Prabhu

Affiliations

  • Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, Pennsylvania, United States.
  • Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, Pennsylvania, United States; Animal Diagnostic Laboratory, Mammalian Pathology Section, Department of Veterinary and Biomedical Sciences. The Pennsylvania State University, University Park, Pennsylvania, United States.
  • Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, Pennsylvania, United States.
  • Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, Pennsylvania, United States.

Abstract

BACKGROUND

Selenium (Se) is an essential trace element that exerts most biological activities through selenoproteins. Dietary selenium is a key regulator of red cell homeostasis and stress erythropoiesis. However, it is unknown whether the form and increasing doses of Se supplementation in the diet impact stress erythropoiesis under anemic conditions.

OBJECTIVES

If inorganic (sodium selenite; Na 2SeO 3) or organic [seleno-L-methionine (Se-Met)] forms of Se in different amounts (deficient, adequate, supplemented, and supranutritional) support stress erythropoiesis in anemic mice.

METHODS

Three-wk-old male C57BL/6 mice were subjected to graded amounts of Se in the form of <0.01 mg/kg Se [Se-deficiency (Se-D)], 0.1 mg/kg Na 2SeO 3 (adequacy), 0.4 mg/kg Na 2SeO 3 (supplemented), 3 mg/kg Na 2SeO 3 (supranutritional), 0.4 mg/kg Se-Met (supplemented), or 3 mg/kg Se-Met (supranutritional), for 10-12 wk before intraperitoneal phenylhydrazine administration to induce hemolytic anemia. Following 3 d of phenylhydrazine injection, spleen and blood samples were used to assess the impact of form and graded amounts of Se in the diet on stress erythropoiesis.

RESULTS

Phenotypic parameters showed that supplementing the diet with Se in the form of Na 2SeO 3 or Se-Met alleviated hemolytic anemia and promoted stress erythropoiesis by supporting the formation of erythroblastic islands. Se-Met at 0.4 mg/kg enhanced erythroid progenitor differentiation by 2-fold compared with Se-D, while Na 2SeO 3 at 0.4 mg/kg and 3 mg/kg significantly (P < 0.05) aided monocyte recruitment and macrophage differentiation within erythroblastic islands. Additionally, 3 mg/kg of Se-Met triggered a stronger inflammatory response than the same dose of Na 2SeO 3. CONCLUSIONS: While both Se-Met and Na 2SeO 3 effectively aid in stress erythropoiesis, Na 2SeO 3 supplementation effectively support stress erythropoiesis with a minimal inflammatory response, while Se-Met at supranutritional dosage lead to increased inflammation despite its support for stress erythropoiesis. These results indicate diverse mechanisms of action of Se on the alleviation of anemia by stress erythropoiesis, which should be considered for further studies to complement existing therapies.

MeSH

Islands, Anemia, Selenoproteins, Stress, Physiological, Anemia, Hemolytic, Methionine, Diet, Erythropoiesis