Journal Article Epublish 1 citation
PubMed: 39744386 DOI: 10.3389/fmicb.2024.1513474
Recombinant avian metapneumovirus subtype C expressing HA protein of H9N2 avian influenza virus are stable and induce protection.
Front Microbiol 15:1513474
Authors: Yu Guo, Jing Cheng, Shuai Zhang, Yuanyuan Zhang, Yuzhu Zuo, Tao Liu, Yun Wang, Chun Yang, Chunjie Cheng, Jinghui Fan, Haijun Jiang
Affiliations
- The College of Veterinary Medicine, Agricultural University of Hebei, Baoding, China. Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.
- Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.
- The College of Veterinary Medicine, Agricultural University of Hebei, Baoding, China.
- The College of Veterinary Medicine, Agricultural University of Hebei, Baoding, China. Baoding Vocational and Technical College, Baoding, China.
Abstract
To prevent H9N2 avian influenza virus (AIV) and Avian metapneumonovirus/C (aMPV/C) infections, we constructed recombinant aMPV/C viruses expressing the HA protein of H9N2 AIV. In addition, EGFP was inserted into the intermediate non-coding region of P-M protein in the aMPV/C genome using a reverse genetic system. The conditions for rescuing the recombinant virus were enhanced followed by insertion of the H9N2 AIV HA gene into the same location in the aMPV/C. The constructed recombinant virus raMPV/C-HA expressed the H9N2 AIV HA protein and showed good stability. Immunization of chicks with raMPV/C-HA increased the generation of neutralizing antibodies against aMPV and H9N2 AIV for 21 days. In the late challenge experiment, raMPV/C-HA effectively inhibited the replication of the virus , decreased the incidence of infection and conferred protection effects.
MeSH
Genes, Influenza In Birds, Influenza, Human, Metapneumovirus, Viruses, Orthomyxoviridae, Infections