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Publications

Journal Article Epublish

PubMed: 39797107 DOI: 10.3390/jcm14010024

The Association Between Periodontal Inflamed Surface Area (PISA), Inflammatory Biomarkers, and Mitochondrial DNA Copy Number.

J Clin Med 14(1)

Authors: Romana Mance Kristan, Staša Jurgec, Uroš Potočnik, Marko Marhl, Rok Gašperšič

Affiliations

  • Community Health Centre dr. Adolf Drolc Maribor, Ulica talcev 9, 2000 Maribor, Slovenia. Department of Periodontology, Faculty of Medicine, University of Maribor, Taborska ulica 8, 2000 Maribor, Slovenia.
  • Centre for Human Genetics & Pharmacogenomics, Faculty of Medicine, University of Maribor, Taborska ulica 8, 2000 Maribor, Slovenia.
  • Laboratory for Biochemistry, Molecular Biology & Genomics, Faculty of Chemistry and Chemical Engineering, University of Maribor, Smetanova ulica 17, 2000 Maribor, Slovenia.
  • Department for Science and Research, University Medical Centre Maribor, Ljubljanska ulica 5, 2000 Maribor, Slovenia.
  • Department of Biophysics, Faculty of Medicine, University of Maribor, Taborska ulica 8, 2000 Maribor, Slovenia.
  • Faculty of Education, University of Maribor, Koroška cesta 160, 2000 Maribor, Slovenia.
  • Faculty of Natural Sciences and Mathematics, University of Maribor, Koroška cesta 160, 2000 Maribor, Slovenia.
  • Department of Oral Medicine and Periodontology, Faculty of Medicine, University of Ljubljana, Hrvatski trg 6, 1000 Ljubljana, Slovenia.

Abstract

: Periodontitis is an inflammatory disease induced by bacteria in dental plaque that can activate the host's immune-inflammatory response and invade the bloodstream. We hypothesized that a higher periodontal inflamed surface area (PISA) is associated with higher levels of inflammatory biomarkers, lower levels of antioxidants, and mitochondrial DNA copy number (mtDNAcn). : Using periodontal parameters, we calculated the PISA score, measured the levels of inflammatory biomarkers and antioxidants in the serum, and took buccal swabs for mtDNA and nuclear DNA (nDNA) extraction. : Higher PISA was associated with higher CRP levels, higher leukocyte, neutrophil, and erythrocyte counts, and lower magnesium-to-calcium ratio, but not with mtDNAcn. A higher number of deep pockets was associated with higher leukocytes and neutrophil counts and higher uric acid levels. : The PISA score might be an appropriate parameter to assess the inflammatory burden of periodontitis, but not to assess mitochondrial dysfunction after mtDNA isolation from buccal swabs.

MeSH

Neutrophils, Biomarkers, Antioxidants, Dna, Mitochondrial, Leukocytes, Dna