Journal Article Research Support, Non-U.S. Gov't Ppublish
PubMed: 39889477 DOI: 10.1016/j.virol.2025.110421
Mapping novel antigenic determinants on foot-and-mouth disease virus serotype Asia 1 using neutralising monoclonal antibody resistant mutants.
Virology 604:110421
Authors: Rajendran Manikandan, Sivarama Krishna Gollapalli, Gopinath Shreya, Suresh Basagoudanavar, B P Sreenivasa, Samriddhi Dubey, V Bhanuprakash, Aniket Sanyal, Mana Mahapatra, Satya Parida, Madhusudan Hosamani
Affiliations
- ICAR-Indian Veterinary Research Institute, Hebbal campus, Bengaluru, 560024, Karnataka, India.
- ICAR-Indian Veterinary Research Institute, Hebbal campus, Bengaluru, 560024, Karnataka, India; ICAR-National Institute of High Security Animal Diseases, Bhopal, 462021, India.
- The Pirbright Institute, Ash Road, Pirbright, Woking, Surrey, GU24 0NF, United Kingdom.
- The Pirbright Institute, Ash Road, Pirbright, Woking, Surrey, GU24 0NF, United Kingdom; Food and Agriculture Organization of the United Nations (FAO), Viale delle Terme di Caracalla, 00153, Rome, Italy.
- ICAR-Indian Veterinary Research Institute, Hebbal campus, Bengaluru, 560024, Karnataka, India.
Abstract
Foot-and-mouth disease (FMD) is a severe and, highly contagious viral disease caused by FMD virus (FMDV) that primarily targets ungulates. Identifying neutralising antigenic sites on the virus is crucial for understanding the virus's antigenic characteristics. Numerous studies have pinpointed the critical residues of the neutralising antigenic sites in FMDV serotypes O, A, and C. However, information on serotype Asia 1 is very limited. This study reports the generation and characterization of forty-one monoclonal antibodies (mAbs) against FMDV serotype Asia 1 (current Indian vaccine strain). Of these, a panel of 23 neutralising mAbs was utilized to generate mAb resistant (MAR) mutants for mapping the virus's antigenic sites. New critical residues defining sites 1, 2, and 4 in FMDV serotype Asia 1 were identified. These findings significantly enhance our understanding of the antigenic nature of FMDV serotype Asia 1, and provide insights to designing broadly acting vaccines against FMD.
MeSH
Foot-And-Mouth Disease, Epitopes, Mouth Diseases, Vaccines, Virology, Foot, Foot-And-Mouth Disease Virus, Viruses, Antibodies, Monoclonal, Mouth, Virus Diseases