Journal Article Review Epublish
PubMed: 39942552 DOI: 10.3390/molecules30030446
Form and Function: The Factors That Influence the Efficacy of Nanomaterials for Gene Transfer to Plants.
Molecules 30(3)
Authors: Zhila Osmani, Marianna Kulka
Affiliations
- Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, AB T6G 2E1, Canada.
- Quantum and Nanotechnologies Research Center, National Research Council Canada, Edmonton, AB T6G 2M9, Canada.
Abstract
Nanoparticle (NP)-mediated gene delivery offers a promising alternative to traditional methods in plant biotechnology, facilitating genetic transformations with enhanced precision and efficiency. This review discusses key factors influencing NP efficacy, including plant cell wall composition, DNA/NP ratios, exposure time, cargo loading, and post-transformation assessments. We explore the challenges of NP cytotoxicity, transformation efficiency, and regeneration while addressing environmental impacts and regulatory considerations. We emphasize the potential for stimulus-responsive NPs and scalable delivery methods to optimize gene editing in agriculture.
MeSH
Genes, Nanoparticles, Nanostructures