Journal Article Research Support, Non-U.S. Gov't Ppublish 1 citation
PubMed: 39978327 DOI: 10.1111/irv.70082
Molecular Analysis of Human Respiratory Syncytial Virus Group B Strains Isolated in Kenya Before and During the Emergence of Pandemic Influenza A/H1N1.
Influenza Other Respir Viruses 19(2):e70082
Authors: Julia Wangui, George Gachara, Victor Mobegi, Charles Agoti, James Otieno, Silvanos Opanda, Benjamin Opot, Joseph N Ngeranwa, Regina Njeru, Wallace Bulimo
Affiliations
- Centre For Virus Research, Kenya Medical Research Institute (KEMRI), Nairobi, Kenya.
- Department of Biochemistry, Kenyatta University, Nairobi, Kenya.
- Department of Medical Laboratory Sciences, Kenyatta University, Nairobi, Kenya.
- Department of Biochemistry, University of Nairobi, Nairobi, Kenya.
- Department of Epidemiology and Demography, Kenya Medical Research Institute (KEMRI) - Wellcome Trust Program, Nairobi, Kenya.
- Theiagen Genomics, Highlands Ranch, Colorado, USA.
- International Livestock Research Institute, Nairobi, Kenya.
Abstract
BACKGROUND
We conducted a retrospective study to explore molecular insights into human respiratory syncytial virus (HRSV) group B strains among patients attending outpatient clinics at government medical facilities both prior and during the onset of Influenza A/H1N1/2009 pandemic outbreak.
METHODS
We screened 2300 nasopharyngeal swabs using multiplex real time reverse transcriptase polymerase chain reaction. We amplified a segment of the first and second hypervariable regions, as well as the conserved portion of the third domain of the G-gene using HRSV-B specific primers, sequenced by Sanger di-deoxy chain termination method and thereafter analyzed the sequences.
RESULTS
We characterized the circulating strains into three known genotypes: SAB4 (1.4%), BA7 (1.4%), and multiple variants of BA9 (97.2%). The majority of BA9 viruses were uniquely Kenyan with only 4% aligning with BA9 lineages found elsewhere. The mean evolutionary rate of the HRSV-B was estimated to be 3.08 × 10 -3 substitutions per site per year.
CONCLUSION
Our findings indicate that the circulating HRSV-B viruses in Kenya underwent a slower evolution during the period of 2007-2010. Additionally, our findings reveal the existence of a unique lineage as well as new variants that have not been reported elsewhere to date.
MeSH
Respiratory Syncytial Viruses, Recombination, Genetic, Influenza, Human, Viruses, Pandemics, Respiratory Syncytial Virus, Human