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Publications

Journal Article Epublish 1 citation

PubMed: 39979331 DOI: 10.1038/s41467-025-57053-9

Peroxisomal core structures segregate diverse metabolic pathways.

Nat Commun 16(1):1802

Authors: Nils Bäcker, Julia Ast, Domenica Martorana, Christian Renicke, Jil Berger, Cristopher-Nils Mais, Marvin Christ, Thorsten Stehlik, Thomas Heimerl, Valentin Wernet, Christof Taxis, Jan Pané-Farré, Michael Bölker, Judith M Klatt, Björn Sandrock, Kay Oliver Schink, Gert Bange, Johannes Freitag

Affiliations

  • Center for Synthetic Microbiology (SYNMIKRO), Philipps-University Marburg, Marburg, Germany. Department of Biology, Philipps-University Marburg, Marburg, Germany.
  • Department of Biology, Philipps-University Marburg, Marburg, Germany.
  • Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA.
  • Center for Synthetic Microbiology (SYNMIKRO), Philipps-University Marburg, Marburg, Germany. Department of Chemistry, Philipps-University Marburg, Marburg, Germany.
  • Center for Synthetic Microbiology (SYNMIKRO), Philipps-University Marburg, Marburg, Germany.
  • Department of Chemistry, Philipps-University Marburg, Marburg, Germany.
  • Department of Microbiology, Institute for Applied Biosciences, KIT, Karlsruhe, Germany.
  • Health and Medical University Erfurt, Erfurt, Germany.
  • Microcosm Earth Center, Philipps-University Marburg & Max-Planck-Institute for terrestrial Microbiology, Marburg, Germany.
  • Centre for Cancer Cell Reprogramming, Faculty of Medicine, University of Oslo, Montebello, Oslo, Norway.
  • Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital, Montebello, Oslo, Norway.
  • Department of Molecular Medicine, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Oslo, Norway.
  • Center for Synthetic Microbiology (SYNMIKRO), Philipps-University Marburg, Marburg, Germany.
  • Department of Chemistry, Philipps-University Marburg, Marburg, Germany.
  • Max-Planck-Institute for terrestrial Microbiology, Molecular Physiology of Microbes, Marburg, Germany.
  • Center for Synthetic Microbiology (SYNMIKRO), Philipps-University Marburg, Marburg, Germany.
  • Department of Biology, Philipps-University Marburg, Marburg, Germany.

Abstract

Peroxisomes are single membrane-bounded oxidative organelles with various metabolic functions including β-oxidation of fatty acids. Peroxisomes of many species confine certain metabolic enzymes into sub-compartments sometimes visible as electron dense cores. Why these structures form is largely unknown. Here, we report that in the smut fungus Ustilago maydis detergent resistant core structures are enriched for different enzymes excluding several key enzymes of the β-oxidation pathway. This confinement contributes to generation of peroxisome subpopulations that differ in their enzyme content. We identify short amino acid motifs necessary and sufficient for protein self-assembly into aggregates in vitro. The motifs trigger enrichment in cores in vivo and are active in mammalian cells. Perturbation of core assembly via variation of such motifs affects peroxisome function in U. maydis strains challenged with fatty acids. Thus, protein core structures serve to compartmentalize the lumen of peroxisomes thereby preventing interference of biochemical reactions. Metabolic compartmentalization of peroxisomes via assembly of specific proteins may occur in other organisms as well.

MeSH

Biological Science Disciplines, Peroxisomes, Enzymes, Fatty Acids

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