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Publications

Journal Article Review Ppublish 14 citations

PubMed: 40037530 DOI: 10.1098/rsob.240231

Deciphering respiratory viral infections by harnessing organ-on-chip technology to explore the gut-lung axis.

Open Biol 15(3):240231

Authors: Hristina Koceva, Mona Amiratashani, Parastoo Akbarimoghaddam, Bianca Hoffmann, Gaukhar Zhurgenbayeva, Mark S Gresnigt, Vanessa Rossetto Marcelino, Christian Eggeling, Marc Thilo Figge, Maria-João Amorim, Alexander S Mosig

Affiliations

  • Institute of Biochemistry II, Jena University Hospital, Jena, Germany.
  • Cluster of Excellence Balance of the Microverse, Friedrich-Schiller-University Jena, Jena, Germany.
  • Applied Systems Biology, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute (HKI), Jena, Germany.
  • Leibniz Institute of Photonic Technologies e.V. Member of the Leibniz Centre for Photonics in Infection Research (LPI), Jena, Germany.
  • Junior Research Group Adaptive Pathogenicity Strategies, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute (HKI), Jena, Germany.
  • Melbourne Integrative Genomics, School of BioSciences, University of Melbourne, Parkville, Australia.
  • Department of Microbiology and Immunology, The Peter Doherty Institute, University of Melbourne, Parkville, Australia.
  • Institute of Applied Optics and Biophysics, Friedrich-Schiller-University Jena, Jena, Germany.
  • Jena Center for Soft Matter, Jena, Germany.
  • Católica Biomédical Research Centre, Católica Medical School, Universidade Católica Portuguesa, Lisbon, Portugal.
  • Center of Sepsis Control and Care, Jena University Hospital, Jena, Germany.

Abstract

The lung microbiome has recently gained attention for potentially affecting respiratory viral infections, including influenza A virus, respiratory syncytial virus (RSV) and SARS-CoV-2. We will discuss the complexities of the lung microenvironment in the context of viral infections and the use of organ-on-chip (OoC) models in replicating the respiratory tract milieu to aid in understanding the role of temporary microbial colonization. Leveraging the innovative capabilities of OoC, particularly through integrating gut and lung models, opens new avenues to understand the mechanisms linking inter-organ crosstalk and respiratory infections. We will discuss technical aspects of OoC lung models, ranging from the selection of cell substrates for extracellular matrix mimicry, mechanical strain, breathing mechanisms and air-liquid interface to the integration of immune cells and use of microscopy tools for algorithm-based image analysis and systems biology to study viral infection . OoC offers exciting new options to study viral infections across host species and to investigate human cellular physiology at a personalized level. This review bridges the gap between complex biological phenomena and the technical prowess of OoC models, providing a comprehensive roadmap for researchers in the field.

MeSH

Cells, Respiratory Syncytial Viruses, Lung, Viruses, Infections, Virus Diseases

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