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Publications

Journal Article Preprint Epublish 3 citations

PubMed: 40060446 DOI: 10.1101/2025.02.24.639159

Comprehensive analysis of myeloid reporter mice.

bioRxiv

Authors: Yidan Wang, Samuel D Dowling, Vanessa Rodriguez, Jessica Maciuch, Meghan Mayer, Tyler Therron, Tovah N Shaw, Miranda G Gurra, Caroline L Shah, Hadijat-Kubura M Makinde, Florent Ginhoux, David Voehringer, Cole A Harrington, Toby Lawrence, John R Grainger, Carla M Cuda, Deborah R Winter, Harris R Perlman

Affiliations

  • Northwestern University, Feinberg School of Medicine. Department of Medicine, Division of Rheumatology. Chicago, IL 60611, USA.
  • Northwestern University, Feinberg School of Medicine. Department of Medicine, Division of Rheumatology. Chicago, IL 60611, USA. Northwestern University, Feinberg School of Medicine. Department of Pediatrics, Division of Rheumatology. Chicago, IL 60611, USA.
  • Institute of Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, Edinburgh, UK.
  • Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR). 8A Biomedical Grove IMMUNOS Bldg, Level 3, SINGAPORE 138648.
  • University Hospital Erlangen, Department of Infection Biology and Friedrich-Alexander University Erlangen-Nuremberg (FAU). Wasserturmstrasse 3-5, 91054 Erlangen, Germany.
  • The Ohio State University Wexner Medical Center, Department of Neurology, The Neuroscience Research Institute, College of Medicine, Columbus, OH, USA.
  • King's College London, Centre for Inflammation Biology and Cancer Immunology, School of Immunology and Microbial Sciences, London, UK.
  • Lydia Becker Institute of Immunology and Inflammation, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, The University of Manchester; Manchester, UK.
  • Center for Human Immunobiology (CHI), Feinberg School of Medicine, Chicago, IL 60611, USA.

Abstract

Macrophages are a pivotal cell type within the synovial lining and sub-lining of the joint, playing a crucial role in maintaining homeostasis of synovium. Although fate-mapping techniques have been employed to differentiate synovial macrophages from other synovial myeloid cells, no comprehensive study has yet been conducted within the mouse synovial macrophage compartment. In this study, we present, for the first time, lineage tracing results from 18 myeloid-specific fate-mapping models in mouse peripheral blood (PB) and synovial tissue. The identification of synovial macrophages and monocyte-lineage cells through flow cytometry was further validated using cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq) datasets. These findings provide a valuable methodological tool for researchers to select appropriate models for studying the function of synovial myeloid cells and serve as a reference for investigations in other tissue types.

MeSH

Cells, Macrophages, Myeloid Cells, Tissues