Journal Article Preprint Epublish 5 citations
PubMed: 40060643 DOI: 10.1101/2025.02.22.639658
Luminos: open-source software for bidirectional microscopy.
bioRxiv
Authors: Daniel G Itkis, F Phil Brooks, Hunter C Davis, Raphael Hotter, J David Wong-Campos, Yitong Qi, Bill Z Jia, Madeleine Howell, Marley Xiong, Rebecca Frank Hayward, Byung Hun Lee, Yangdong Wang, Rebecca T Perelman, Adam E Cohen
Affiliations
- Department of Chemistry and Chemical Biology, Harvard University, Cambridge MA 02138.
Abstract
Bidirectional microscopy (BDM) combines simultaneous targeted optical perturbation and imaging of biophysical or biochemical signals (e.g. membrane voltage, Ca, or signaling molecules). A core challenge in BDM is precise spatial and temporal alignment of stimulation, imaging, and other experimental parameters. Here we present Luminos, an open-source MATLAB library for modular and precisely synchronized control of BDM experiments. The system supports hardware-triggered synchronization across stimulation, recording, and imaging channels with microsecond accuracy. Source code and documentation for Luminos are available online at https://www.luminosmicroscopy.com and https://github.com/adamcohenlab/luminos-microscopy. This library will facilitate development of bidirectional microscopy methods across the biological sciences.
MeSH
Microscopy, Software