Journal Article Epublish 2 citations
PubMed: 40072131 DOI: 10.3390/nano15050328
Aqueous Extract Mediated Silver Nanoparticles: Part I. Influence of Synthesis Parameters, Characterization, and Biomedical Studies.
Nanomaterials (Basel) 15(5)
Authors: Himabindu Kurra, Aditya Velidandi, Mounika Sarvepalli, Ninian Prem Prashanth Pabbathi, Vikram Godishala
Affiliations
- Department of Biotechnology, Vaagdevi Degree and P.G. College, Warangal 506001, India. Department of Biotechnology, Bharatiya Engineering Science and Technology Innovation University, Gorantla 515231, India.
- Department of Biotechnology, National Institute of Technology, Warangal 506004, India.
Abstract
This study explores the green synthesis of silver nanoparticles (AgNPs) using (lemongrass) extract as a reducing agent. Synthesis was confirmed by a color change (light yellow to dark brown) under optimal conditions: 1.50 mM silver nitrate, 3.5% / extract, at 100 °C, with a pH of 9, and for 60 min. The AgNPs exhibited spherical morphology, a hydrodynamic diameter of 135.41 ± 49.30 nm, a zeta potential of -29.9 ± 1.4 mV, crystalline structure, and minimal aggregation. AgNPs showed significant antibacterial activity, particularly at >20 µg/well, with the zones of inhibition varying by bacterial strain. In vitro studies demonstrated anti-inflammatory, antidiabetic (α-glucosidase and α-amylase inhibition), and antioxidant activities, with AgNPs outperforming plant extract and nearing standard efficacy at higher concentrations. Cyto-toxicity studies indicated that AgNPs and plant extract were less toxic than doxorubicin but exhibited concentration-dependent effects on cancerous and non-cancerous cells. Eco-toxicity assays revealed that AgNPs were less acutely toxic than controls but posed risks with prolonged exposure. This work highlights the eco-friendly synthesis of AgNPs and their potential in biomedical applications, demonstrating efficacy in antibacterial and antioxidant activities.
MeSH
Plant Extracts, Nanoparticles, Antioxidants