Journal Article Epublish
PubMed: 40076862 DOI: 10.3390/ijms26052240
Anti-Hair Loss Effect of Veratric Acid on Dermal Papilla Cells.
Int J Mol Sci 26(5)
Authors: Jiyoung You, Youngsu Jang, Junbo Sim, Dehun Ryu, Eunae Cho, Deokhoon Park, Eunsun Jung
Affiliations
- Biospectrum Life Science Institute, Sinsu-ro, Suji-gu, Yongin-City 16827, Gyeonggi-Do, Republic of Korea.
Abstract
The activation of hair follicle dermal papilla cells (HFDPCs), a critical target of hair loss relief, can be achieved through the upregulation of proliferation, the stimulation of hair inducibility, and the inhibition of cellular senescence. Veratric acid (VA) is a major benzoic acid found in fruits and vegetables. The biological activity of VA on HFDPCs remains to be elucidated. In this study, we investigated the capacity of VA for hair loss mitigation. An MTT assay, Ki67 staining, quantitative RT-PCR (qRT-PCR), and a Western blot analysis were performed to confirm the proliferative effect of VA. Hair inductivity was determined through a cell aggregation assay and ALP staining. Annexin V/PI staining was performed to confirm the anti-apoptotic effect of VA. The inhibitory effect of VA on cellular senescence was confirmed by a β-galactosidase (β-gal) assay and qRT-PCR using replicative senescence and oxidative stress-induced senescence models. As a result, VA dose-dependently upregulated the proliferation of HFDPCs, the expression of growth factors, and β-catenin protein levels. VA also dose-dependently increased ALP activity and cell aggregation and decreased apoptotic cells through the regulation of and expression. Moreover, VA reduced β-gal activity and the senescence-associated secretory phenotype (SASP) in a dose-dependent manner in senescent HFDPCs. These findings suggest that VA may serve as a potential therapeutic agent for alleviating hair loss by targeting multiple pathways involved in HFDPC activation.
MeSH
Cells, Catenins, Cell Aggregation, Cellular Senescence, Alopecia