Journal Article Epublish
PubMed: 40077572 DOI: 10.3390/foods14050870
Supplementation with Lentil () Hull Soluble Dietary Fiber Ameliorates Sodium Dextran Sulfate-Induced Colitis and Behavioral Deficits via the Gut-Brain Axis.
Foods 14(5)
Authors: Dongying Chen, Xin Bi, Qian Feng, Yong Sun
Affiliations
- The Second Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang 330006, China. State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, China.
- State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, China.
- The Second Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang 330006, China.
Abstract
In this study, the impact of lentil hull soluble dietary fibers (SDFs) on colitis and behavioral deficits in mice was assessed. Structural characterizations of SDFs confirmed that cellulase-modified soluble dietary fiber exhibited better physicochemical properties: more porous microstructure; similar polysaccharide structure; more stable particle size distribution; higher crystallinity; better adsorption capacity; and lower viscosity. Additionally, we explored its potential cognitive benefits via the gut-brain axis by behavioral tests, histopathology, 16S rRNA sequencing, gas chromatography and metabolomics analysis. The results showed that SDFs significantly improved inflammatory symptoms in colon and brain and cognitive behaviors. LSDF had better efficacy than HSDF. LSDF intervention decreased the harmful bacteria abundance (, and , etc.) and increased beneficial bacteria abundance ( and , etc.). LSDF also affected brain metabolites through the sphingolipid metabolism. Spearman correlation analysis showed that there was a positive correlation between harmful bacteria with inflammatory factors (LPS, IL-1β, IL-6, and TNF-α, etc.) and sphingolipid metabolites, while beneficial bacteria were positively correlated with brain-derived neurotrophic factor (BDNF), IL-10, and cognitive behavior. This study highlights the value of SDFs in future diet-based therapeutic strategies targeting gut-brain interactions.
MeSH
Sphingolipids, Brain-Derived Neurotrophic Factor, Colitis, Brain, Cognitive Dysfunction, Diet, Cognition Disorders, Dextran Sulfate, Dietary Fiber, Bacteria