Journal Article Epublish
PubMed: 40109533 DOI: 10.1364/BOE.555679
Adaptive lensless microscopic imaging with unknown phase modulation.
Biomed Opt Express 16(3):1160-1171
Authors: Xiangyu Chen, Hao Sha, Chunyu Chen, Yuan Jiang, Wenzhen Zou, Yongbing Zhang
Affiliations
- School of Computer Science and Technology, Harbin Institute of Technology, Shenzhen, Guangdong 518055, China.
- School of Computer Science and Technology, Harbin Institute of Technology, Harbin 150001, China.
Abstract
Lensless imaging is a popular research field because of its small size, wide field-of-view, and low aberration in recent years. However, some traditional lensless imaging methods suffer from slow convergence, mechanical errors, and conjugate solution interference, which limit their further application and development. In this work, we proposed a lensless imaging method based on a spatial light modulator (SLM) with unknown phase modulation values. In our imaging system, the SLM is utilized to modulate the wavefront of the object. When the phase modulation values of the SLM are inaccurate or unknown, conventional algorithms such as amplitude-phase retrieval (APR) or the extended ptychographic iterative engine (ePIE) fail to reconstruct the complex amplitude information of the object. To address this challenge, we introduce a novel approach that combines ptychographic scanning along a spiral path with the ePIE algorithm, enabling accurate reconstruction of the original image. We further analyze the effect of modulation function and the characteristics of the coherent light source on the quality of the reconstructed image. The experiments show that the proposed method is superior to traditional methods in terms of recovering speed and accuracy, with the recovering resolution up to 14 in the reconstruction of the USAF phase plate image.
MeSH
Algorithms, Solutions