Journal Article Epublish
PubMed: 40109536 DOI: 10.1364/BOE.544498
Increasing the spatial bandwidth product in light field microscopy with remote scanning.
Biomed Opt Express 16(3):1062-1070
Authors: Aymerick Bazin, Amaury Badon
Affiliations
- LP2N, Laboratoire Photonique Numéerique et Nanosciences, Univ. Bordeaux, F-33400 Talence, France. Institut d'Optique Graduate School & CNRS UMR 5298, F-33400 Talence, France.
- LP2N, Laboratoire Photonique Numéerique et Nanosciences, Univ. Bordeaux, F-33400 Talence, France.
- Institut d'Optique Graduate School & CNRS UMR 5298, F-33400 Talence, France.
Abstract
Achieving fast, large-scale volumetric imaging with micrometer resolution has been a persistent challenge in biological microscopy. To address this challenge, we report an augmented version of light field microscopy, incorporating a motorized tilting mirror upstream of the camera. Depending on the scanning pattern, the field of view and/or the lateral resolution can be greatly improved. Our microscope technique is simple, versatile, and configured for bright-field and epifluorescence modes. We demonstrate its performance with imaging of multi-cellular aggregates of various shapes and sizes.
MeSH
Microscopy