Journal Article Epublish 3 citations
PubMed: 40114978 DOI: 10.1155/bca/2009069
An Innovative Approach Based on the Green Synthesis of Silver Nanoparticles Using Pomegranate Peel Extract for Antibacterial Purposes.
Bioinorg Chem Appl 2025:2009069
Authors: Rocío Díaz-Puertas, Francisco J Álvarez-Martínez, Enrique Rodríguez-Cañas, Fernando Borrás, Artur J M Valente, José A Paixao, Alberto Falcó, Ricardo Mallavia
Affiliations
- Institute of Research, Development and Innovation in Healthcare Biotechnology in Elche (IDiBE), Miguel Hernández University, Elche 03202, Alicante, Spain.
- Statistics and Operative Research Department, Miguel Hernández University, Elche 03202, Alicante, Spain.
- Department of Chemistry, University of Coimbra, Coimbra 3004-535, Portugal.
- Department of Physics, University of Coimbra, Coimbra 3004-516, Portugal.
- Fish Pathology Group, Institute of Aquaculture Torre de La Sal-Spanish National Research Council (IATS-CSIC), Cabanes 12595, Castellón, Spain.
Abstract
This study describes a green synthesis method for silver nanoparticles (AgNPs) using autochthonous "Mollar de Elche" pomegranate peel extract and optimized through a Python-programmed Box-Behnken design (BBD) created specifically for the work. The bioactive compounds in pomegranate, particularly punicalagin, serve as effective reducing and stabilizing agents. BBD was used to analyze the effects of dependent variables such as silver nitrate concentration, pomegranate extract concentration, and temperature on responses such as hydrodynamic diameter, polydispersity index, and zeta potential, minimizing experimental trials and highlighting variable interactions. Optimal conditions were experimentally validated and agreed well with the predicted values. The optimized AgNPs were characterized via ultraviolet-visible spectrophotometry, Fourier transform infrared spectroscopy, X-ray diffraction, and field emission scanning electron microscopy. These AgNPs demonstrated substantial antibacterial activity against and . Furthermore, the AgNPs were incorporated into nanofibrous scaffolds as a proof of concept for potential biomedical applications, where their antibacterial activity was partially retained postincorporation. This study highlights the potential of pomegranate extract as a sustainable medium for AgNP synthesis with promising antibacterial applications and the ability of the BBD as a useful tool for efficient optimization of multivariable processes, including the synthesis of nanomaterials.
MeSH
Nanoparticles