Journal Article Epublish
PubMed: 40115251 DOI: 10.1002/fsn3.70105
Fermentation Broths Attenuate Bleomycin-Induced Pulmonary Fibrosis by Activating the Nrf2/HO-1/NQO1 Signaling Pathway and Modulating Gut Microbiota.
Food Sci Nutr 13(3):e70105
Authors: Heting Zhou, Xinyue Zheng, Shaolin Huang, Xiaomeng Wang, Ting Zhou, Shuwen Zhang, Yihan Ling, Wenxi Wang, Xingjie Li, Shouqian Li, Yongmei Xie, Wenya Yin
Affiliations
- West China School of Public Health and West China Fourth Hospital, Sichuan University Chengdu China.
- State Key Laboratory of Biotherapy and Cancer Center West China Hospital, Sichuan University Chengdu China.
- Department of Clinical Nutrition Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China Chengdu China.
- Guizhou Jinqianguo Biotechnology Co. Ltd. Bijie China.
Abstract
Pulmonary fibrosis (PF) is a chronic and progressive lung disease, and oxidative stress plays a critical role in its pathogenesis. Tratt, known for its anti-inflammatory and antioxidant properties, has been shown to alleviate fibrosis. This study aimed to explore whether two fermentation broths (RRFBs) (with different proportions) could attenuate bleomycin (BLM)-induced PF in mice and to elucidate the molecular mechanisms. The results revealed that RRFBs reduced structural lung damage, collagen deposition, and lung inflammation. RRFBs also suppressed fibrotic markers (Collagen I, Vimentin, and α-SMA) while enhancing epithelial marker E-cadherin expression. Additionally, RRFBs alleviated BLM-induced oxidative stress and apoptosis by activating the Nrf2/HO-1/NQO1 signaling pathway and facilitating Nrf2 nuclear translocation. Furthermore, RRFBs attenuated the BLM-induced changes in the gut microbiota; in particular, they decreased the abundance of the pathogenic bacterium and increased the abundance of the probiotics and . Spearman correlation analysis revealed a strong association between oxidative stress inhibition and gut microbiota composition. These results indicated that RRFBs could exert lung-protective effects by inhibiting oxidative stress and alleviating intestinal disturbances.
MeSH
Bleomycin, Lung, Fibrosis, Stress, Physiological, Oxidative Stress, Pulmonary Fibrosis, Gastrointestinal Microbiome, Microbiota