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Publications

Journal Article Ppublish

PubMed: 40128392 DOI: 10.1038/s41593-025-01921-6

Neural ensembles that encode nocifensive mechanical and heat pain in mouse spinal cord.

Nat Neurosci 28(5):1012-1023

Authors: Ming-Dong Zhang, Jussi Kupari, Jie Su, Kajsa A Magnusson, Yizhou Hu, Laura Calvo-Enrique, Dmitry Usoskin, Gioele W Albisetti, Mikaela M Ceder, Katharina Henriksson, Andrew D Leavitt, Hanns Ulrich Zeilhofer, Tomas Hökfelt, Malin C Lagerström, Patrik Ernfors

Affiliations

  • Department of Medical Biochemistry and Biophysics, Division of Molecular Neurobiology, Karolinska Institutet, Stockholm, Sweden.
  • Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden.
  • Department of Cell Biology and Pathology, Instituto de Neurociencias de Castilla y León (INCyL), Universidad de Salamanca, Salamanca, Spain.
  • Institute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland. Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology (ETH) Zurich, Zurich, Switzerland.
  • Department of Medicine, University of California, San Francisco, CA, USA.
  • Department of Laboratory Medicine, University of California, San Francisco, CA, USA.
  • Institute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland.
  • Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology (ETH) Zurich, Zurich, Switzerland.
  • Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden.
  • Department of Medical Biochemistry and Biophysics, Division of Molecular Neurobiology, Karolinska Institutet, Stockholm, Sweden.

Abstract

Acute pain is an unpleasant experience caused by noxious stimuli. How the spinal neural circuits attribute differences in quality of noxious information remains unknown. By means of genetic capturing, activity manipulation and single-cell RNA sequencing, we identified distinct neural ensembles in the adult mouse spinal cord encoding mechanical and heat pain. Reactivation or silencing of these ensembles potentiated or stopped, respectively, paw shaking, lifting and licking within but not across the stimuli modalities. Within ensembles, polymodal Gal + inhibitory neurons with monosynaptic contacts to A-fiber sensory neurons gated pain transmission independent of modality. Peripheral nerve injury led to inferred microglia-driven inflammation and an ensemble transition with decreased recruitment of Gal + inhibitory neurons and increased excitatory drive. Forced activation of Gal + neurons reversed hypersensitivity associated with neuropathy. Our results reveal the existence of a spinal representation that forms the neural basis of the discriminative and defensive qualities of acute pain, and these neurons are under the control of a shared feed-forward inhibition.

MeSH

Spinal Cord, Neurons, Pain, Spine