Journal Article Epublish
PubMed: 40129648 DOI: 10.1039/d5ra00519a
Box-Behnken optimized MPA-CdTe quantum dots as turn-off fluorescent probes for sensitive lurasidone determination in pharmaceutical, biological, and environmental matrices.
RSC Adv 15(12):8855-8866
Authors: Razaz Abdulaziz Felemban, Maram H Abduljabbar, Reem M Alnemari, Rami M Alzhrani, Yusuf S Althobaiti, Mohammed F Aldawsari, Ahmed Serag, Atiah H Almalki
Affiliations
- Department of Basic Medical Sciences, College of Medicine, King Saud Bin Abdulaziz University for Health Sciences Jeddah Saudi Arabia.
- King Abdullah International Medical Research Centre Jeddah Saudi Arabia.
- Department of Pharmacology and Toxicology, College of Pharmacy, Taif University P. O. Box 11099 Taif 21944 Saudi Arabia.
- Department of Pharmaceutics and Industrial Pharmacy, College of Pharmacy, Taif University P. O. Box 11099 Taif 21944 Saudi Arabia.
- Addiction and Neuroscience Research Unit, Health Science Campus, Taif University P. O. Box 11099 Taif 21944 Saudi Arabia.
- Department of Pharmaceutics, College of Pharmacy, Prince Sattam Bin Abdulaziz University Al-kharj 11942 Saudi Arabia.
- Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University Nasr City 11751 Cairo Egypt.
- Department of Pharmaceutical Chemistry, College of Pharmacy, Taif University P. O. Box 11099 Taif 21944 Saudi Arabia.
Abstract
A sensitive and selective fluorescence quenching method was developed for the determination of lurasidone using MPA-CdTe quantum dots as a "turn-off" fluorescent probe. The fluorescence intensity of the MPA-CdTe QDs was quenched upon the addition of lurasidone, with the quenching efficiency exhibiting a linear relationship with the lurasidone concentration in the range of 0.02-1.0 μg mL. Stern-Volmer analysis revealed that the quenching mechanism was predominantly static in nature, and thermodynamic studies indicated that the interaction between lurasidone and MPA-CdTe QDs was exothermic and spontaneous in nature. Factors affecting the quenching process, including pH, MPA-CdTe QDs volume, and incubation time, were optimized using a Box-Behnken experimental design. A significant model was obtained with a coefficient of determination ( ) of 0.9547, demonstrating the reliability of the optimization process. The analytical performance of the method was validated according to ICH guidelines, exhibiting good linearity and sensitivity with LOD of 5.90 ng mL and LOQ of 17.70 ng mL. The accuracy and precision of the method were assessed through recovery studies, showing satisfactory results with a mean recovery of 98.65 ± 0.733% and RSD% > 2%. The proposed method was successfully applied to the analysis of lurasidone in pharmaceutical dosage forms, spiked plasma, and environmental water samples, with good recoveries and precision. The greenness and analytical practicality of the method were evaluated using AGREE and BAGI tools, respectively, and the results showed that the proposed method is a greener and more practical alternative to previously reported analytical techniques for the determination of lurasidone. The present study demonstrates the potential of MPA-CdTe QDs as a sensitive and selective fluorescent probe for the determination of lurasidone in various matrices, with good analytical performance and environmental compatibility.
MeSH
Pharmaceutical Preparations, Recycling, Quantum Dots