Journal Article Epublish 7 citations
PubMed: 40135239 DOI: 10.3389/fphar.2025.1543594
Zingerone alleviates inflammatory pain by reducing the intrinsic excitability of anterior cingulate cortex neurons in a mice model.
Front Pharmacol 16:1543594
Authors: Yuanyuan Wang, Lang Dong, Shu Han, Yuehan You, Mingrui Zhang, Bingjing Sun, Hong Ni, Rongjing Ge, Jianhong Liu, Jiandong Yu
Affiliations
- Department of Neurosurgery, the First Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui, China.
- Laboratory of Brain and Psychiatric Disease, Bengbu Medical University, Bengbu, Anhui, China.
- School of Basic Medicine, Bengbu Medical University, Bengbu, Anhui, China.
- Department of Neurosurgery, the First Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui, China. Laboratory of Brain and Psychiatric Disease, Bengbu Medical University, Bengbu, Anhui, China.
- Laboratory of Brain and Psychiatric Disease, Bengbu Medical University, Bengbu, Anhui, China. School of Basic Medicine, Bengbu Medical University, Bengbu, Anhui, China.
- Laboratory of Brain and Psychiatric Disease, Bengbu Medical University, Bengbu, Anhui, China. Department of Biochemistry and Molecular biology, School of Laboratory Medicine, Bengbu Medical University, Bengbu, Anhui, China.
- Department of Neurosurgery, the First Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui, China. Laboratory of Brain and Psychiatric Disease, Bengbu Medical University, Bengbu, Anhui, China. Anhui Engineering Research Center for Neural Regeneration Technology and Medical New Materials, Bengbu Medical University, Bengbu, Anhui, China.
Abstract
Background
has been shown to possess analgesic properties. Zingerone (ZO), a bioactive compound derived from Zingiber officinale Roscoe, exhibits a range of pharmacological effects, including anti-inflammatory, anti-cancer, antioxidant, antibacterial, and anti-apoptotic activities. However, the analgesic properties of zingerone remain unclear.
Methods
Complete Freund's adjuvant (CFA) was administered to the left hind paw of C57BL/6 mice to induce a model of inflammatory pain. The analgesic effects of zingerone were assessed using the Von Frey and Hargreaves tests. fiber photometry and whole-cell patch clamp techniques were employed to investigate the potential mechanisms.
Results
Both acute and long-term treatment with zingerone resulted in a significant increase in mechanical and thermal pain thresholds in mice experiencing CFA-induced inflammatory pain. Mechanical stimulation led to a pronounced increase in calcium levels within the anterior cingulate cortex (ACC) neurons of the inflammatory pain model, which was alleviated by zingerone administration. Furthermore, zingerone was found to modify synaptic transmission to ACC neurons and decrease their intrinsic excitability by prolonging the refractory period of these neurons.
Conclusion
Zingerone demonstrates potential for alleviating CFA-induced inflammatory pain by reducing the intrinsic excitability of ACC neurons in a mouse model.
MeSH
Neurons, Pain, Analgesics, Synaptic Transmission