Journal Article Epublish
PubMed: 40161410 DOI: 10.1002/fsn3.70121
Exploring the Utility of Extracts as a Source of Natural Bioactive Compounds for Functional Applications.
Food Sci Nutr 13(4):e70121
Authors: Bayram Atasagun, Ahmet Uysal, Noha Fathallah, Omayma Eldahshan, Abdel Nasser Singab, Mehmet Veyis Cetiz, Gokhan Zengin
Affiliations
- Department of Medical Services and Techniques Vocational School of Health Services, Selcuk University Konya Türkiye.
- Department of Pharmacognosy and Medicinal Plants, Faculty of Pharmacy Future University in Egypt Cairo Egypt.
- Department of Pharmacognosy, Faculty of Pharmacy Ain Shams University Cairo Egypt.
- Faculty of Pharmacy, Center for Drug Discovery Research and Development Ain Shams University Cairo Egypt.
- Department of Medical Biochemistry, Faculty of Medicine Harran University Sanliurfa Turkey.
- Department of Biology, Science Faculty Selcuk University Konya Turkey.
Abstract
has garnered attention as a potent medicinal agent and functional component. We aimed to detect the chemical composition and biological activities of several parts (fruit, leaves, and twigs) of . Biological activities were assessed for antioxidant properties, enzyme inhibition, mutagenic/antimutagenic effects, and antibacterial efficacy. Antioxidant capabilities were evaluated using various assays, including DPPH, ABTS, CUPRAC, FRAP, phosphomolybdenum, and metal chelating. The chemical constituents of the extracts were identified and quantified using the HPLC-ESI-MS/MS method. The effects of enzyme inhibition were examined on some enzymes, including AChE, BChE, tyrosinase, amylase, and glucosidase. The Ames test was used to evaluate the mutagenic and antimutagenic properties of the plant extracts. Furthermore, a broth microdilution assay was employed to evaluate the possible antibacterial effects of the extracts against microorganisms. The methanol extract of twigs showed superior antioxidant capabilities (DPPH: 388.39 mg TE/g; ABTS: 701.50 mg TE/g; CUPRAC: 459.05 mg TE/g; FRAP: 264.99 mg TE/g). The methanol extract of twigs demonstrated the highest tyrosinase inhibitory activity (61.91 mg KAE/g). A total of 40 metabolites, mainly flavonoids, were detected through HPLC-ESI-MS/MS analysis, revealing that ferulic acid, naringenin, and herniarin were the predominant compounds. In the Ames test, the tested extracts exhibited no mutagenic potential. The antimutagenicity assay demonstrated that methanol and ethyl acetate extracts from twigs and leaves were particularly efficient against frameshift and base pair substitution mutations induced by recognized mutagens. The metabolic activation system amplified these strong activities to inhibition rates ranging from 85% to 98%. The results from the antibacterial assay indicated antibacterial effectiveness at dosages between 6.25 and 0.195 mg/mL, particularly effective against , and . Our findings indicate that can serve as a versatile raw material for the development of health-promoting applications, including medicines, cosmeceuticals, and nutraceuticals.
MeSH
Methanol, Monophenol Monooxygenase, Antioxidants, Enzymes