Journal Article Epublish
PubMed: 40177422 DOI: 10.1515/biol-2022-1030
Inhibition of mast cell activation by Jaranol-targeted Pirin ameliorates allergic responses in mouse allergic rhinitis.
Open Life Sci 20(1):20221030
Authors: Yue Huang, Shuhua Su, Bo Duan, Yunfei Zhang, Zhengmin Xu
Affiliations
- Department of Otolaryngology-Head and Neck Surgery, Children's Hospital of Fudan University, No 399 Wanyuan Road, Shanghai, China.
- Department of Otolaryngology, Qinzhou Maternity and Child Health Care Hospital, Qinzhou, Guangxi Province, China.
Abstract
Jaranol, a bioactive compound derived from various traditional medicinal herbs, has demonstrated significant anti-inflammatory properties. This study investigates its effects and possible mechanisms underlying its anti-inflammatory role in mast cells, as well as ovalbumin (OVA)-induced allergic rhinitis (AR) mice model. Forty mice were randomly divided into blank, AR, dexamethasone (positive control), and Jaranol groups (10 mg/ml), with 10 mice in each group. Jaranol was found to inhibit nasal mucosal inflammation and reduce mast cell numbers in AR models. It also inhibited the secretion of several inflammatory cytokines (IFN-γ, TNF-α, IL-1β, IL-6, MCP-1, and CXCR10) from mast cells, as well as mast cell proliferation and migration. Interestingly, Pirin was differentially expressed in blank, AR, and Jaranol groups. Further studies indicated that Jaranol inhibited inflammatory cytokine secretion from mast cells by mediating Pirin and also inhibited mast cell proliferation and migration. Moreover, it inhibited mast cell function by suppressing Pirin expression. These findings suggest that Jaranol exerts its therapeutic effects by inhibiting Pirin expression in mast cells, thereby reducing inflammation and histopathological changes associated with AR.
MeSH
Cytokines, Cells, Cell Proliferation, Rhinitis, Allergic, Rhinitis, Mast Cells