Journal Article Epublish
PubMed: 40225128 DOI: 10.1016/j.mtbio.2025.101708
Macrophage membrane-coated polydopamine nanomedicine for treating acute lung injury through modulation of neutrophil extracellular traps and M2 macrophage polarization.
Mater Today Bio 32:101708
Authors: Yuwei Zhao, Xingyu Zhu, Letao Hu, Fangyu Hao, Xianglei Ji, Xiaofang Hu, Meimei Luo, Linyu Zheng, Bo Xiao, Yingmei Wu, Changcan Shi, Hui Zhu, Nong Zhou, Weidong Li
Affiliations
- School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China.
- Chongqing College of Traditional Chinese Medicine, Chongqing, 402760, China.
- Chongqing Three Gorges University, Chongqing, 402020, China.
Abstract
Acute lung injury (ALI) is a life-threatening pulmonary inflammatory disorder with high morbidity and mortality rates. Effective targeting of damaged lung tissues and regulation of inflammatory dysregulation are major challenges in clinical treatment. This study aimed to develop a multifunctional drug delivery system by coating mesoporous polydopamine nanoparticles (mPDA NPs) loaded with Peimine (PM) using macrophage membranes (MMs) to leverage their inflammatory targeting properties. Both in vitro and in vivo experiments demonstrated the excellent targeting capability, strong antioxidant activity, and significant anti-inflammatory effects of the developed MM@mPDA-PM NPs. Furthermore, transcriptomics analysis revealed that MM@mPDA-PM NPs significantly reduced myeloperoxidase (MPO), neutrophil elastase (NE), and peptidylarginine deiminase 4 (PAD4), as well as inhibited the formation of neutrophil extracellular traps (NETs), and promoted M2 macrophage polarization by downregulating the NF-κB and JAK/STAT pathways. Our developed system effectively reduced neutrophil infiltration, suppressed cytokine storms, and regulated the pulmonary immune microenvironment, demonstrating great potential for treating ALI and other inflammatory diseases.
MeSH
Lung, Nanomedicine, Acute Lung Injury, Macrophages, Lung Injury, Neutrophils, Extracellular Traps, Membranes