Journal Article Epublish
PubMed: 40271454 DOI: 10.1093/hr/uhaf058
Molecular mechanism of activates the expression of , thereby modulating tanshinone accumulation in .
Hortic Res 12(6):uhaf058
Authors: Xinyu Wang, Yifei Shi, Qichao Wang, Xinjia Xie, Siqi Gui, Jiening Wu, Limei Zhao, Xiaowei Zou, Guoyin Kai, Wei Zhou
Affiliations
- Laboratory for Core Technology of TCM Quality Improvement and Transformation, School of Pharmaceutical Sciences, School of Pharmacy and Academy of Chinese Medical Science, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Abstract
Tanshinones are bioactive diterpenoid chemicals of the herb with a characteristic furan D-ring. As a newly identified downstream enzyme, SmCYP71D375, catalyzes hydroxylation by 14,16-ether (hetero)cyclization to form the furan D-ring from the precursor of the phenolic abietane-type diterpenoids that exist widely in Lamiaceae plants. However, its transcriptional regulatory network, with as the direct target gene, remains unclear. In the present study, the promoter of was employed as the bait to mine the upstream regulatory protein using the cDNA yeast library of . An R2R3-MYB transcription factor gene, , was identified. Overexpressing in transgenic hairy roots upregulated expression, thereby accelerating tanshinone accumulation, whereas tanshinone accumulation was inhibited in RNAi transgenic hairy root lines. To dissect the regulatory network of , was captured using as the bait to prey for its potential interacting proteins in the cDNA yeast library. Yeast two-hybrid, glutathione -transferase pull-down, and bimolecular fluorescence complementation assays were independently used to verify the interaction between the SmMYB53 and SmbZIP51 proteins . We further verified that the upregulation of activated by SmMYB53 would be inhibited by the interaction of SmMYB53 and SmbZIP51. The present findings uncover the molecular regulatory network underlying as the direct target regulating tanshinone biosynthesis and offer a basis for the genetic improvement of medicinal substance biosynthesis in .
MeSH
Genes, Yeasts, Diterpenes