Journal Article Comparative Study Epublish
PubMed: 40299876 DOI: 10.1371/journal.pone.0322268
Chronic active and chronic inactive hepatitis B virus infection: Comparative study of genetic polymorphism and blood profile measures.
PLoS One 20(4):e0322268
Authors: Khalid Elyass Awadelkarim, Najem Aldin M Osman, A M S Eleragi, Abdelsalam M A Nail, Nadir Abuzeid, Mohamed E Elangeeb, Elsadig Mohamed Ahmed, Jaber Ahmed Al-Faifi, Abdullah Alhalafi, Ahmed S Doghish, Osama A Mohammed
Affiliations
- Department of Microbiology, Faculty of Medical Laboratory Sciences, Omdurman Islamic University, Khartoum, Sudan.
- Department of Microorganisms and Clinical Parasitology, College of Medicine, University of Bisha, Bisha, Saudi Arabia.
- Department of Internal Medicine, Faculty of Medicine, Omdurman Islamic University, Khartoum, Sudan.
- Tropical Diseases Teaching Hospital, Khartoum, Sudan.
- Department of Microbiology, Medical Laboratory Sciences program, Delta College of Science and Technology, Sudan.
- Department of Basic Medical Sciences, College of Applied Medical Sciences, University of Bisha, Bisha, Saudi Arabia.
- Department of Medical Laboratory Sciences, College of Applied Medical Sciences, University of Bisha, Bisha, Saudi Arabia.
- Department of Child Health, College of Medicine, University of Bisha, Bisha, Saudi Arabia.
- Department of Family and Community Medicine, College of Medicine, University of Bisha, Bisha, Saudi Arabia.
- Department of Biochemistry, Faculty of Pharmacy, Badr University in Cairo, Cairo, Egypt.
- Department of Biochemistry and Molecular Biology, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo, Egypt.
- Department of Pharmacology, College of Medicine, University of Bisha, Bisha, Saudi Arabia.
Abstract
INTRODUCTION
Hepatitis B virus (HBV) is a significant cause of chronic hepatitis, leading to liver complications such as cirrhosis and hepatocellular carcinoma. Toll-like receptors (TLRs) are critical in the immune response to HBV. This study investigates the TLR3 1377 C/T polymorphism's association with clinical outcomes in chronic hepatitis B patients.
MATERIALS AND METHODS
A case-control study included 136 participants (66 cases and 70 controls). The patients were categorized as having chronic active or inactive hepatitis B based on serological, biochemical, and molecular parameters. TLR3 1377 C/T polymorphism was analyzed using PCR-RFLP. The correlation between TLR3 genotypes, HBeAg status, liver enzyme levels (ALT and AST), and symptomatic presentation was assessed.
RESULTS
Among the 66 cases, the CC genotype was more frequent in males with chronic active hepatitis (14 males, 5 females), but no significant gender-based difference was observed in genotype distribution. A significant association was found between the CC genotype and symptomatic presentation in chronic active cases (P = 0.015). However, no significant association was detected between TLR3 genotypes and HBeAg status (P > 0.05). Elevated ALT and AST levels were more prevalent in chronic active cases.
CONCLUSIONS
The TLR3 1377 C/T polymorphism, particularly the CC genotype, may influence the clinical presentation of chronic hepatitis B, particularly in symptomatic cases. However, its impact on HBeAg status and overall chronicity appears limited. Further studies are needed to clarify the role of TLR3 polymorphisms in HBV pathogenesis and their potential as therapeutic targets.
MeSH
Blood, Hepatitis B Virus, Herpesviridae Infections, Hepatitis, Chronic, Liver, Herpesvirus 1, Cercopithecine, Viruses, Polymorphism, Genetic, Genotype, Hepatitis B, Infections, Hepatitis, Virus Diseases