Journal Article Ppublish
PubMed: 40329779 DOI: 10.1002/brb3.70499
Unraveling the Role of α2δ-1 in Cerebral Hemorrhage: Calcium Overload, Endoplasmic Reticulum Stress, and Microglial Apoptosis.
Brain Behav 15(5):e70499
Authors: Ning Yu, Xiaopeng Li, Bingqian Wang, Chengrui Nan, Qianxu Jin, Liang Yang, Depei Li, Zongmao Zhao
Affiliations
- Department of Anesthesiology and intensive care unit, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, China.
- Department of Neurosurgery, The First Hospital of Handan City, Handan, Hebei Province, China.
- Department of Neurosurgery, Affiliated Xing Tai People Hospital of Hebei Medical University, Xingtai, Hebei Province, China.
- Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, China.
- Department of Medicine, University of Missouri School of Medicine One Hospital Drive, Columbia, Missouri, USA.
Abstract
OBJECTIVE
Cerebral hemorrhage is a severe condition associated with high morbidity and mortality. Understanding the underlying pathogenesis is crucial for developing effective therapeutic strategies. This study aimed to investigate the role of the dysregulated α2δ-1 protein in cerebral hemorrhage.
MATERIALS AND METHODS
We observed a significant upregulation of α2δ-1 in cerebral hemorrhage tissue. Knockdown of α2δ-1 resulted in decreased intracellular calcium concentration and reduced phosphorylation of PLCr and IP3R in the presence of calcium. Additionally, α2δ-1-mediated calcium overload induced ERS in BV2 microglia, accompanied with increased phosphorylation of PERK and decreased ERS-related protein levels.
RESULTS
α2δ-1 knockdown significantly inhibited BV2 microglia apoptosis and downregulated apoptosis-related proteins in the presence of calcium. Our study indicates the involvement of α2δ-1 in calcium-mediated signaling, endoplasmic reticulum stress, and BV2 microglia apoptosis.
CONCLUSIONS
The findings provide a basis for considering α2δ-1 as a potential therapeutic target in cerebral hemorrhage and secondary brain injury conditions associated with calcium dysregulation.
MeSH
Microglia, Cerebral Hemorrhage, Hemorrhage, Stress, Physiological, Endoplasmic Reticulum Stress, Calcium, Endoplasmic Reticulum, Therapeutics, Apoptosis, Calcium Signaling