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Publications

Journal Article Epublish

PubMed: 40331939 DOI: 10.3390/ijms26083499

Plasma Microvesicles May Contribute to Muscle Damage in the Mouse Model of Duchenne Muscular Dystrophy.

Int J Mol Sci 26(8)

Authors: Cynthia Machado Cascabulho, Samuel Iwao Maia Horita, Daniela Gois Beghini, Rubem Figueiredo Sadok Menna-Barreto, Ana Carolina Heber Max GuimarĂ£es Monsores, Alvaro Luiz Bertho, Andrea Henriques-Pons

Affiliations

  • LaboratĂ³rio de Inovações em Terapias, Ensino e Bioprodutos, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro 21040-360, RJ, Brazil.
  • LaboratĂ³rio de Pesquisas Sobre o Timo, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro 21040-360, RJ, Brazil.
  • LaboratĂ³rio de Biologia Celular, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro 21040-360, RJ, Brazil.
  • LaboratĂ³rio de Imunoparasitologia, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro 21040-360, RJ, Brazil.

Abstract

Extracellular vesicles (EVs) are cell-derived lipid-bound vesicles divided into apoptotic bodies, microvesicles (MVs), and exosomes based on their biogenesis, release pathway, size, content, and functions. EVs are intercellular mediators that significantly affect muscle diseases such as Duchenne muscular dystrophy (DMD). DMD is a fatal X-linked disorder caused by mutations in the dystrophin gene, leading to muscle degeneration. mice are the most commonly used model to study the disease, and in this study, we phenotypically characterized plasma MVs from mice by flow cytometry. Furthermore, we assessed the ability of plasma MVs to modulate muscle inflammation, damage, and/or regeneration by intramuscular injection of MVs from mice into or DBA/2 mice as a control. In both mouse lineages, platelets and erythrocytes were the primary sources of MVs, and CD3 CD4 MVs were observed only in mice. We also observed that plasma MVs from mice induced muscle damage in mice but not in DBA/2 mice, while plasma MVs from DBA/2 mice did not induce muscle damage in either mouse lineage. These results indicate that plasma MVs from are potentially pathogenic. However, this condition also depends on the muscular tissue status, which must be responsive due to active inflammatory or regenerative responses.

MeSH

Cells, Genes, Extracellular Vesicles, Muscular Dystrophies, Muscles, Muscular Dystrophy, Duchenne, Plasma