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Publications

Journal Article Epublish

PubMed: 40332541 DOI: 10.3390/ijms26083819

Neuronally Derived Extracellular Vesicles' Oligomeric and p129-α-Synuclein Levels for Differentiation of Parkinson's Disease from Essential Tremor.

Int J Mol Sci 26(8)

Authors: Costanza Maria Cristiani, Selena Mimmi, Elvira Immacolata Parrotta, Mariagrazia Talarico, Anna Maria Tolomeo, Elisabetta Pingitore, Khushboo Fatima, Basilio Vescio, Luana Scaramuzzino, Valentina Crapella, Anna Maria Zimbo, Enrico Iaccino, Giovanni Cuda, Aldo Quattrone, Andrea Quattrone

Affiliations

  • Neuroscience Research Center, Department of Medical and Surgical Sciences, University "Magna Graecia", 88100 Catanzaro, Italy.
  • Laboratory of Stem Cells, Department of Medical and Surgical Sciences, University "Magna Graecia", 88100 Catanzaro, Italy.
  • Institute of Pediatric Research Città della Speranza, 35128 Padua, Italy.
  • Department of Cardiac, Thoracic and Vascular Science and Public Health, University of Padova, 35128 Padua, Italy.
  • Department of Experimental and Clinical Medicine, University "Magna Graecia", 88100 Catanzaro, Italy.
  • Biotecnomed S.c.ar.l. 88100 Catanzaro, Italy.
  • IBSBC-CNR, Via T. Campanella, 115, 88100 Catanzaro, Italy.
  • Institute of Neurology, Department of Medical and Surgical Sciences, University "Magna Graecia", 88100 Catanzaro, Italy.

Abstract

Clinical differentiation between Parkinson's disease (PD) and essential tremor (ET) may be challenging, highlighting the need for easily assessable diagnostic biomarkers. Neuronally derived extracellular vesicles (NDEVs) have been proposed as a peripheral matrix that can well recapitulate the cellular composition of neurons. We investigated the clinical usefulness of NDEV oligomeric and p129-α-synuclein levels in discriminating between patients with PD and those with ET. NDEV oligomeric and p129-α-synuclein species were assessed using an ELISA in 43 patients with PD, 21 patients with ET, and 45 healthy controls (HCs). NDEV oligomeric α-synuclein levels were significantly higher in PD in comparison with ET and HCs, while p129-α-synuclein values were significantly lower in HCs compared to other groups. By using a receiver operator characteristic (ROC) analysis, oligomeric-α-synuclein achieved an excellent classification performance in distinguishing PD from both ET and HCs (AUC: 0.976 and 0.997, respectively), while lower performance was obtained in differentiating ET from HCs (AUC: 0.85). On the other hand, p129-α-synuclein accurately discriminated both PD and ET from HCs (AUC: 0.997 and 0.952, respectively) but had very low performance in differentiating PD from ET (AUC: 0.47). Our study suggests that NDEV oligomeric α-synuclein is an accurate blood-derived biomarker to differentiate PD from ET, while p129-α-synuclein may be useful in distinguishing ET from HCs.

MeSH

Extracellular Vesicles, Essential Tremor, Parkinson Disease, Tremor, Biomarkers, Synucleins