Journal Article Epublish
PubMed: 40362031 DOI: 10.3390/ani15091217
Cloning and Transcriptional Activity Analysis of the Bovine CDH11 Gene Promoter: Transcription Factors Sp1 and GR Regulate Bovine CDH11 Expression.
Animals (Basel) 15(9)
Authors: Zhanxin Liu, Yanbin Bai, Zongchang Chen, Yanmei Niu, Xue Jia, Liang Li, Xiaolan Zhang, Binggang Shi, Zhidong Zhao, Jiang Hu, Yuzhu Luo, Jiqing Wang, Xiu Liu, Shaobin Li, Fangfang Zhao
Affiliations
- Gansu Provincial Key Laboratory of Herbivore Biotechnology, College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China.
Abstract
Growth rate of bovine skeletal muscle has a major impact on beef yield. Cadherin-11 (CDH11) was found to be a potential candidate gene for growth and development in beef cattle. This study confirmed the high expression of CDH11 in bovine longissimus dorsi using a tissue expression analysis. To understand the transcriptional regulation mechanism of CDH11 gene, we constructed a double luciferase vector for the promoter region of CDH11 gene and determined that the core transcriptional regulatory region was located at -129/+55 bp relative to the transcription start site (TSS). In addition, we confirmed that skeletal muscle growth and development-related transcription factor-specific protein 1 (SP1) and glucocorticoid receptor (GR) bind to the CDH11 gene promoter region at -36/-27 bp and -20/-11 bp, respectively, to regulate CDH11 expression. These interactions provide valuable information for understanding the mechanism of transcriptional regulation of the bovine CDH11 gene during muscle growth and development.
MeSH
Genes, Growth, Promoter Regions, Genetic, Muscles, Transcription Factors, Muscle, Skeletal, Growth And Development