Journal Article Epublish
PubMed: 40427537 DOI: 10.3390/biom15050644
Serine Hydroxymethyltransferase Modulates Midgut Physiology in Aedes aegypti Through miRNA Regulation: Insights from Small RNA Sequencing and Gene Expression Analysis.
Biomolecules 15(5)
Authors: Qian Pu, Yujiao Han, Zhuanzhuan Su, Houming Ren, Qingshan Ou, Symphony Kashyap, Shiping Liu
Affiliations
- State Key Laboratory of Resource Insects, Southwest University, Chongqing 400715, China.
Abstract
Aedes aegypti mosquitoes are critical vectors of arboviruses, responsible for transmitting pathogens that pose significant public health challenges. Serine hydroxymethyltransferase (SHMT), a key enzyme in one-carbon metabolism, plays a vital role in various biological processes, including DNA synthesis, energy metabolism, and cell proliferation. Although SHMT is expressed at low levels in the midgut of Aedes aegypti, its silencing has been shown to inhibit blood meal digestion. The precise mechanisms by which SHMT regulates midgut physiology in mosquitoes remain poorly understood. In this study, we employed small RNA sequencing and quantitative PCR to identify differentially expressed miRNAs (DEMs) following SHMT downregulation. We focused on a subset of DEMs-miR-2940-5p, miR-2940-3p, miR-2941, and miR-306-5p-to explore their potential biological functions. To further elucidate the molecular mechanisms underlying the miRNA response to SHMT downregulation, we analyzed the expression levels of key genes involved in the miRNA biogenesis pathway. Our results demonstrated that several critical enzymes, including Drosha, Dicer1, and AGO1, exhibited significant changes in expression upon SHMT silencing. This study provides new insights into the molecular mechanisms through which SHMT influences the biological functions and nutritional metabolism of the mosquito midgut. By linking SHMT activity to miRNA regulation, our findings highlight a potential pathway by which SHMT modulates midgut physiology, offering a foundation for future research into mosquito biology and vector control strategies.
MeSH
Genes, Physiology, Micrornas, Enzymes, Down-Regulation, Glycine Hydroxymethyltransferase, Serine, Gene Expression, Rna