Journal Article Epublish
PubMed: 40429657 DOI: 10.3390/ijms26104510
Lycium ruthenicum Murray Anthocyanins Alleviate Aging Through SIRT1/P53 Signaling Pathway.
Int J Mol Sci 26(10)
Authors: Jialin Liang, Zang Ga, Jiaqin Wu, Yingjie Wang, Nanjia Dongzhu, Rangzhong Qieyang, Ping Li, Sangduo Huaqian
Affiliations
- College of Ecological Environment Engineering, Qinghai University, Xining 810016, China.
- College of Tibetan Medicine, Qinghai University, Xining 810016, China.
- Medical Department, Qinghai University, Xining 810016, China.
- College of Ecological Environment Engineering, Qinghai University, Xining 810016, China. State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 810016, China.
Abstract
Aging-related diseases have become a global health issue, with the escalating aging population leading to an increased disease incidence, placing immense pressure on individual health and society. Lycium ruthenicum Murray anthocyanins are hailed as the "Black Pearl of the Desert". Anthocyanins are potent natural antioxidants that can combat oxidation, reduce inflammation, prevent cardiovascular diseases, protect the liver, and inhibit tumor cell growth. As individuals age, the accumulation of free radicals in the body accelerates aging. Antioxidants mitigate aging by neutralizing free radicals, and the anthocyanins in Lycium ruthenicum Murray effectively reduce oxidative damage, activate the antioxidant enzyme system, and enhance the body's antioxidant capacity, thereby slowing the aging process. This study investigated Lycium ruthenicum Murray Anthocyanins' (LRAs) anti-aging mechanisms using D-galactose-induced H9c2 cells and H 2O 2-treated zebrafish. LRAs increased survival rates (30.47% cells, 20.02% zebrafish), reduced ROS, Sa-β-gal, and apoptosis markers, while boosting antioxidant enzymes (SOD, CAT, GSH) and lowering MDA. It upregulated Bcl-2/SIRT1 and downregulated Bax/P53/P21/NF-κB/MAPK/TNF-α genes, with protein-level SIRT1 activation and P53/P21 suppression. The transcriptome analysis revealed a significant reduction in aging-related gene expression levels. The results demonstrated that LRAs mitigate aging through SIRT1/P53-mediated oxidative stress inhibition and apoptosis reduction, suggesting their therapeutic potential for age-related disorders.
MeSH
Cells, Genes, Aging, Antioxidants, Genes, P53, Enzymes, Galactose, Free Radicals, Apoptosis, Sirtuin 1, Anthocyanins