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Publications

Journal Article Epublish

PubMed: 40457360 DOI: 10.1186/s12917-025-04848-z

Integrative transcriptomic and proteomic analyses reveal that carbon metabolism and complement system of Madin Darby Bovine Kidney cells are affected by bovine coronavirus infection.

BMC Vet Res 21(1):398

Authors: Hui Zhang, Qisheng Yu, Rui Guo, Qing Zhu, Jin Yu, Zhaohui Zhang, Lan Lan, Cheng Tang, Changqing Yu, Bin Zhang

Affiliations

  • College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, 610041, China.
  • Center for Animal Disease Control and Prevention, Ganzi Tibetan Autonomous Prefectue, Kangding, 626000, China.
  • Animal Husbandry Science Institute of Ganzi Tibetan Autonomous Prefecture, Kangding, 626000, China.
  • Engineering Center of Agricultural Biosafety Assessment and Biotechnology, School of Advanced Agricultural Sciences, Yibin Vocational and Technical College, Yibin, 644001, China.
  • College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, 610041, China. Key Laboratory of Ministry of Education and Sichuan Province for Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Chengdu, 610041, China.

Abstract

BACKGROUND

Bovine coronavirus (BCoV) is a major pathogen of bovine respiratory disease, causing respiratory and enteric infections in cattle and wild ruminants. It is responsible for economic losses and threatens the health and welfare of cattle industry.

RESULTS

In this study, a BCoV isolate, BCoV/SUWN/XHD-5, had cytopathogenic effects in Madin Darby bovine kidney (MDBK) cells and showed a high viral titer at 24 and 48 h post infection (hpi). Gene expression profiling using RNA sequencing and protein network mapping using the Tandem Mass Tag-based quantitative proteomics approach were performed in MDBK cells with BCoV infection at 24 and 48 hpi, respectively. Compared with mock-infected MDBK cells, 8,720 differentially expressed genes (DEGs) and 296 differentially expressed proteins (DEPs) were identified in BCoV infection at 24 hpi, whereas 5,838 DEGs and 747 DEPs were identified in BCoV infection at 48 hpi. Following GO annotation and KEGG enrichment analysis, most DEGs and DEPs were significantly enriched in metabolic pathways, endocytosis, ribosome and protein processing in endoplasmic reticulum, apoptosis and immune response. A correlation analysis of the proteome and transcriptome revealed that the up-regulated DEGs and DEPs were predominantly associated with metabolic pathways, apoptosis and the MAPK/TNF/Ras signaling pathway, whereas the down-regulated DEGs and DEPs were involved in complement and coagulation cascades and the Wnt signaling pathway. Importantly, BCoV decreases the mRNA and protein levels of complement component C3 in MDBK cells.

CONCLUSIONS

The findings of this study provide the first report of the integrative transcriptomic and proteomic analyses of BCoV infection in MDBK cells. It revealed a regulatory network for analyzing the mechanisms of BCoV-host interactions and provides valuable insights into the pathogenesis of BCoV.

MeSH

Cells, Coronavirus, Genes, Coronavirus, Bovine, Kidney, Coronavirus Infections, Complement System Proteins, Apoptosis, Infections