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Publications

Journal Article Epublish

PubMed: 40563339 DOI: 10.3390/antiox14060707

Synergistic Effect of TiO 2-Nanoparticles and Plant Growth-Promoting Microorganisms on the Physiological Parameters and Antioxidant Responses of Capsicum annum Cultivars.

Antioxidants (Basel) 14(6)

Authors: Atiya Bhatti, Araceli Sanchez-Martinez, Gildardo Sanchez-Ante, Daniel A Jacobo-Velázquez, Joaquín Alejandro Qui-Zapata, Soheil S Mahmoud, Ghulam Mustafa Channa, Luis Marcelo Lozano, Jorge L Mejía-Méndez, Edgar R López-Mena, Diego E Navarro-López

Affiliations

  • Tecnologico de Monterrey, Escuela de Ingeniería y Ciencias, Ave. General Ramon Corona 2514, Zapopan 45138, Jalisco, Mexico.
  • Departamento de Ingeniería de Proyectos, CUCEI, Universidad de Guadalajara, Av. José Guadalupe Zuno # 48, Industrial los Belenes, Zapopan 45157, Jalisco, Mexico.
  • Tecnologico de Monterrey, Institute for Obesity Research, Ave. General Ramon Corona 2514, Zapopan 45201, Jalisco, Mexico.
  • Biotecnología Vegetal, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C. Camino Arenero 1227, El Bajío, Zapopan 45019, Jalisco, Mexico.
  • Department of Biology, The University of British Columbia, Okanagan Campus, 1177 Research Road, Kelowna, BC V1V 1V7, Canada.
  • Programa de Edafología, Colegio de Postgraduados, Campus Montecillo, Carretera México Texcoco km 36.4, Montecillo 56264, Mexico.

Abstract

Titanium dioxide nanoparticles (TiO 2-NPs) were synthesized using the molten salt method and systematically characterized. TiO 2-NPs were evaluated for their capacity to promote the growth of Capsicum annuum cultivars together with the plant growth-promoting microorganisms (PGPMs) Bacillus thuringiensis (Bt) and Trichoderma harzianum (Th). The variables analyzed included physiological parameters and antioxidant responses. The capacity of TiO 2-NPs to scavenge free radicals was also investigated, along with their biocompatibility, using Artemia salina as an in vivo model. The results demonstrated that TiO 2-NPs exhibited a nanocuboid-type morphology, negative surface charge, and small surface area. It was noted that TiO 2-NPs enhanced the CFU and spore production of Bt (1.56-2.92 × 10 8 CFU/mL) and Th (2.50-3.90 × 10 8 spores/mL), respectively. It was observed that TiO 2-NPs could scavenge DPPH, ABTS, and H 2O 2 radicals (IC 50 48.66-109.94 μg/mL), while not compromising the viability of A. salina at 50-300 μg/mL. TiO 2-NPs were determined to enhance the root length and fresh and dry weights of chili peppers. Similarly, TiO 2-NPs in synergy with Bt and Th increased the activity of β-1,3-Glucanase (2.45 nkat/g FW) and peroxidase (69.90 UA/g FW) enzyme activity, and increased the TPC (29.50 GA/g FW). The synergy of TiO 2-NPs with the PGPMs consortium also upregulated the total chlorophyll content: 210.8 ± 11.4 mg/mg FW. The evidence from this study unveils the beneficial application of TiO 2-NPs with Bt and Th as an efficient approach to promote the physiology and antioxidant responses of chili peppers.

MeSH

Growth, Nanoparticles, Antioxidants, Spores