Journal Article Epublish
PubMed: 40563976 DOI: 10.3390/biology14060726
Isolation and Bioactive Characterization of Berberis kaschgarica Rupr-Derived Exosome-Like Nanovesicles: Exploring Therapeutic Potential in Atherosclerosis Pathogenesis.
Biology (Basel) 14(6)
Authors: Dilihuma Dilimulati, Nuerbiye Nueraihemaiti, Alhar Baishan, Sendaer Hailati, Alifeiye Aikebaier, Yipaerguli Paerhati, Wenting Zhou
Affiliations
- Department of Pharmacology, School of Pharmacy, Xinjiang Medical University, Urumqi 830017, China. Xinjiang Key Laboratory of Natural Medicines Active Components and Drug Release Technology, Urumqi 30017, China. Xinjiang Key Laboratory of Biopharmaceuticals and Medical Devices, Urumqi 830017, China. Engineering Research Center of Xinjiang and Central Asian Medicine Resources, Ministry of Education, Urumqi 830017, China.
- Department of Pharmacology, School of Pharmacy, Xinjiang Medical University, Urumqi 830017, China.
- Xinjiang Key Laboratory of Natural Medicines Active Components and Drug Release Technology, Urumqi 30017, China.
- Xinjiang Key Laboratory of Biopharmaceuticals and Medical Devices, Urumqi 830017, China.
- Engineering Research Center of Xinjiang and Central Asian Medicine Resources, Ministry of Education, Urumqi 830017, China.
Abstract
Berberis kaschgarica Rupr.-derived exosome-like nanovesicles (BELNs), a type of plant-derived extracellular vesicle, consist of proteins, lipids, and nucleic acids. In this research, we employed differential centrifugation and ultracentrifugation techniques to isolate and purify BELNs. Subsequently, we conducted a comprehensive multi-omics analysis to systematically determine their physicochemical properties. Experiments were conducted in vitro with Human Umbilical Vein Endothelial Cells (HUVECs) to verify the therapeutic impact of BELNSs on atherosclerosis. The isolated BELNs exhibited a distinctive teacup-shaped exosome morphology. The extraction yield was approximately 2.1 × 10 13 particles per milliliter, and the average particle size was measured to be 179.1 nm. These nanovesicles were lipid-rich. The protein content predominantly comprised cytoplasmic proteins. In-depth analysis revealed the presence of five highly conserved plant microRNAs: miR166, miR156, miR399, miR171, and miR395. These miRNAs are involved in regulating plant growth and responses to both biotic and abiotic stresses. Functional assays demonstrated that Berberis kaschgarica Rupr.-derived exosome-like nanovesicles substantially decreased the lipid deposition in HUVECs that was triggered by Palmitic Acid (PA). This research establishes the inaugural utilization of multi-omics platforms to systematically elucidate the bioactivity profile of BELNs from Berberis kaschgarica Rupr., thereby laying the groundwork for advancing its therapeutic potential.
MeSH
Micrornas, Multiple Chronic Conditions, Exosomes, Proteomics, Research, Atherosclerosis, Therapeutics, Lipidomics