Journal Article Epublish
PubMed: 40572172 DOI: 10.3390/microorganisms13061284
Lactobacillus helveticus R0052 and Bifidobacterium longum R0175 Supplementation: An Exploratory, Randomized, Placebo-Controlled Trial of Endocannabinoid and Inflammatory Responses in Female Dancers.
Microorganisms 13(6)
Authors: Jakub Wiącek, Karolina Skonieczna-Żydecka, Igor Łoniewski, Chariklia K Deli, Ioannis G Fatouros, Athanasios Z Jamurtas, Dominika Moszczyńska, Joanna Karolkiewicz
Affiliations
- Department of Food and Nutrition, Poznan University of Physical Education, 61-871 Poznan, Poland.
- Department of Biochemical Research, Pomeranian Medical University, 70-204 Szczecin, Poland.
- Sanprobi Sp. z o.o. Sp.K. 70-535 Szczecin, Poland.
- Department of Physical Education and Sport Science, University of Thessaly, 38221 Trikala, Greece.
Abstract
The anandamide (AEA) and lipopolysaccharide (LPS) interaction is gaining attention, but evidence on the influence of probiotics on endocannabinoid system (ECS) biomarkers remains limited. This study (NCT05567653) investigated the effects of 12-week supplementation with Lactobacillus helveticus R0052 and Bifidobacterium longum R0175 on AEA (main outcome) and inflammatory biomarkers in female dancers. Fifteen participants (5 probiotic, 10 placebo) were included in the final analysis. Serum levels of AEA, LPS, and cytokines (tumor necrosis factor-alpha-TNF-α, interleukin-1 beta-IL-1β, and interleukin-10-IL-10) were measured using an ELISA (enzyme-linked immunosorbent assay), and the psychological stress responses were evaluated using the Mini-COPE questionnaire. At the baseline, a correlation between AEA and LPS was observed (Spearman's r = 0.9677, p < 0.05). After 12 weeks, no statistically significant differences in the AEA, LPS, cytokine levels, or stress-coping strategies were observed between the probiotic and placebo groups (LPS-probiotic: +3.48 EU/L, p = 0.9361; placebo: +56.98 EU/L, p = 0.0694; AEA-probiotic: -1.11 ng/mL, p = 0.9538; placebo: +14.08 ng/mL, p = 0.4749). The direction of change may indicate a trend toward increased inflammation in the absence of probiotics, consistent with patterns described in previous literature. However, these results should be viewed as hypothesis generating and warrant confirmation in larger trials.
MeSH
Cytokines, Tumor Necrosis Factor-Alpha, Stress, Physiological, Enzyme-Linked Immunosorbent Assay, Biomarkers, Bifidobacterium Longum, Lactobacillus Helveticus, Probiotics, Bifidobacterium, Interleukin-1, Endocannabinoids, Lactobacillus, Gastrointestinal Microbiome, Lipopolysaccharides, Interleukin-10