Journal Article Epublish
PubMed: 40595519 DOI: 10.1038/s41467-025-60897-w
Spatial microfluidic holographic integrated platform for label-free and high-dimensional analysis of cancer heterogeneity.
Nat Commun 16(1):5890
Authors: Jiayi Shi, Huijing Teng, Ziyi Zhang, Yanping Liu, Dan Gao, Jianglei Di, Zijian Yang, Ping Su, Ying Tan, Jianshe Ma
Affiliations
- Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, PR China.
- Institute of Advanced Photonics Technology, School of Information Engineering, Guangdong University of Technology, Guangzhou, PR China.
- Department of Breast and Thyroid Surgery, Peking University Shenzhen Hospital, Shenzhen, PR China.
- Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, PR China.
- Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, PR China.
Abstract
The combination of quantitative phase microscopy (QPM) with imaging flow cytometry (IFC) enables label-free and multi-parameter single-cell analysis. Here, we present a simple yet powerful QPM-IFC platform, the spatial microfluidic holographic integrated (SMHI) platform, which uniquely integrates spatial hydrodynamic focusing microfluidics with digital holographic microscopy (DHM) to achieve high-fidelity single-cell QPM reconstruction without digital refocusing in 0.34 seconds, accounting for only 4.41% of the typical process ( ~ 7.71 seconds). We develop a high-dimensional phase feature hierarchy and implement a maximun-relevance and minimun-redundancy incremental feature selection (MRMR-IFS) strategy, which effectively addresses feature redundancy and constructs the optimal feature set. Consequently, a prediction accuracy of >99.9% is achieved across multiple cancer cell types, breast cancer subtypes, and blood cells, demonstrating its efficacy in analyzing highly heterogeneous cell populations. Notably, this system also exhibits high accuracy in analyzing simulated blood samples, highlighting its great potential in practical applications.
MeSH
Cells, Blood, Neoplasms, Multiple Chronic Conditions, Microfluidics, Microscopy, Quantitative Phase Imaging