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Publications

Journal Article Ahead of print

PubMed: 40626496 DOI: 10.1177/0271678X251355049

2-deoxy-D-glucose chemical exchange-sensitive spin-lock MRI of cerebral glucose metabolism after transient focal stroke in the rat.

J Cereb Blood Flow Metab

Authors: Philipp Boehm-Sturm, Patrick Schuenke, Marco Foddis, Susanne Mueller, Stefan P Koch, Daniel J Beard, Paul Holloway, Amin Mottahedin, Leif Schröder, Alastair M Buchan, Philipp Mergenthaler

Affiliations

  • Charité-Universitätsmedizin Berlin, Center for Stroke Research Berlin, Berlin, Germany.
  • Charité-Universitätsmedizin Berlin, Experimental Imaging at the Charité for 3R (EPIC3R), Berlin, Germany.
  • Charité-Universitätsmedizin Berlin, Department of Neurology with Experimental Neurology, Berlin, Germany.
  • Charité-Universitätsmedizin Berlin, Department of Radiology, Berlin, Germany.
  • Physikalisch-Technische Bundesanstalt (PTB), Braunschweig and Berlin, Germany.
  • School of Biomedical Sciences and Pharmacy, The University of Newcastle, Newcastle, Australia.
  • Radcliffe Department of Medicine, University of Oxford, Oxford, UK.
  • Consortium International pour la Recherche Circadienne sur l'AVC (CIRCA).
  • Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford, UK.
  • German Cancer Research Center, Translational Molecular Imaging, Heidelberg, Germany.
  • Department for Physics and Astronomy, Ruprecht Karls University Heidelberg, Heidelberg, Germany.
  • German Cancer Consortium (DKTK), Heidelberg, Germany.

Abstract

Rapid breakdown of cerebral glucose metabolism is a hallmark in stroke pathology. Metabolic activity delineates the penumbra from the infarct core, representing tissue that is potentially salvageable by therapeutic interventions. Tools to image dynamics of glucose and its spatial distribution could provide biomarkers of disease severity and of the success of therapeutic interventions. Here, we developed a new protocol to measure glucose transport and metabolism in a rat model of stroke using chemical exchange-sensitive spin-lock (CESL) MRI of the glucose analogue 2-deoxy-D-glucose (2DG). We further implemented a protocol that combines 2DG-CESL-MRI with perfusion and diffusion MRI to relate this new signal to established definitions of hypoperfused tissue, cytotoxic edema and the penumbra. We found that 2DG-CESL-MRI provides a biomarker of disturbed glucose transport and metabolism after stroke with high effect size. This is the first study to investigate CESL MRI of 2DG in the context of transport and metabolism imaging in rodent stroke.

MeSH

Deoxyglucose, Stroke, Biomarkers, Therapeutics, Tissues