Journal Article Epublish
PubMed: 40686997 DOI: 10.1021/acsomega.5c02279
Effect of Biogenic Gold Nanoparticles on Gut Microbiota Composition during Larval-to-Pupal Transition in L.
ACS Omega 10(27):29317-29330
Authors: Trishanjan Biswas, Rajarshi Bhattacharya, Rittick Mondal, Halil Kurt, Md Majharul Islam, Shubhajit Shaw, Pankaj Mandal, Paulami Dam, Debnirmalya Gangopadhyay, Azamal Husen, Amiya Kumar Panda, Abdul Sadat, Yavuz Nuri Ertas, Amit Kumar Mandal
Affiliations
- Department of Sericulture, Raiganj University, North Dinajpur, Raiganj, West Bengal 733134, India.
- Centre for Molecular Neuroscience, Kasturba Medical College, Manipal Academy of Higher Education, Manipal 576104, India.
- Department of Medical Biology, Hamidiye International School of Medicine, University of Health Sciences Türkiye, Uskudar, Istanbul 34668, Türkiye.
- Department of Microbiology, Calcutta University, Ballygunge Circular Road, Kolkata 700019, West Bengal, India.
- Wolaita Sodo University, P.O. Box 138, Wolaita Sodo, South Ethiopia Regional State 4620, Ethiopia.
- Department of Biotechnology, Graphic Era (Deemed to be University), Dehradun, Uttarakhand 248002, India.
- Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, West Bengal 721102, India.
- Rani Rashmoni Green University, Tarakeswar,Hooghly 712410, West Bengal, India.
- Department of Biomedical Engineering, Erciyes University, Kayseri 38039, Türkiye. ERNAM-Nanotechnology Research and Application Center, Erciyes University, Kayseri 38039, Türkiye.
- Center for Nanotechnology Sciences (CeNS), Raiganj University, North Dinajpur, Raiganj, West Bengal 733134, India.
Abstract
This study investigates the effects of biogenic gold nanoparticles (Au NPs), synthesized using an aqueous leaf extract of , as a feed supplement on the gut microbiota of . The synthesized Au NPs displayed a characteristic peak absorbance at 545 nm in the UV-vis spectrum, indicative of surface plasmon resonance (SPR), a phenomenon typical of Au NPs. Fourier-transform infrared (FTIR) spectra revealed the functional groups responsible for the reduction of Au-(III) to Au(0). The crystallinity of the synthesized Au NPs was checked using TEM and XRD analysis. TEM micrographs further exhibited the quasi-spherical, monodispersed, well-scattered nature of the Au NPs with an average particle size of 34.15 ± 9.45 nm. The presence of (111), (200), (220), and (311) planes in Bragg's reflections confirmed the face-centered-cubic (fcc) crystalline nature of elemental gold. The LC-MS/MS study revealed that the presence of two zwitterionic species (mainly betaine) stabilizes the Au NPs by the formation of positive sol micelles. Biogenic Au NPs at 40-60 ppm concentrations significantly improved larval growth, effective rearing rate (ERR), filament length, and cocoon quality without affecting silk fineness. Doses below 40 ppm were biologically ineffective, while concentrations above 60 ppm triggered oxidative stress and cytotoxicity. Similarly, gut microbiota analysis revealed notable compositional shifts at the phylum, class, family, and genus levels. There was a decrease in the relative abundance of the phylum Cyanobacteria, concomitant with a significant enrichment of stress-resilient taxa, including Planctomycetes, Micrococcaceae, Parcubacteria, and Candidatus Adlerbacteria. Predictive functional profiling using PICRUSt indicated enhanced microbial pathways linked to metabolism, stress response, and detoxification. These results suggest that biogenic Au NPs influence host-microbiome interactions, facilitating improved nutrient assimilation and silk production. However, further research is required to evaluate the long-term safety and ecological impact before widespread use in sericulture.
MeSH
Nanoparticles, Stress, Physiological, Silk, Gastrointestinal Microbiome, Microbiota