Journal Article Ppublish
PubMed: 40699420 DOI: 10.1007/978-1-0716-4726-4_3
Detection of Cancer-Associated Gene Fusion by Ligation-Dependent RT-PCR.
Methods Mol Biol 2962:25-37
Authors: Vinciane Rainville, Marie-Delphine Lanic, Fanny Drieux, Nicolas Piton, David Rizzo, Fabrice Jardin, Philippe Ruminy
Affiliations
- INSERM U1245, Centre Henri Becquerel, University of Rouen, Rouen, France.
- Department of Pathology, Centre Henri Becquerel, Rouen, France.
- Department of Pathology, CHU Rouen, Rouen, France.
- CNRS UMR-7276, INSERM U1262, Team 1 2MB2C of CRIBL, University of Limoges, and Hematology Laboratory of Dupuytren Hospital University Center (CHU) of Limoges, Limoges, France.
- Department of Hematology, Centre Henri Becquerel, Rouen, France.
- INSERM U1245, Centre Henri Becquerel, University of Rouen, Rouen, France.
Abstract
Ligation-mediated RT-PCR is a rapid and highly sensitive solution to detect the presence of gene fusions in cancers. Here, we provide a detailed protocol which allows the detection of hundreds of these tumor markers in a single assay, in a rapid turnaround time.
MeSH
Genes, Polymerase Chain Reaction, Neoplasms, Gene Fusion, Ligation, Reverse Transcriptase Polymerase Chain Reaction