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Publications

Journal Article Epublish

PubMed: 40699852 DOI: 10.3390/cimb47060453

Miyako Bidens pilosa Extract Ameliorates Allodynia and Suppresses Spinal Microglial Activation in Mice with Partial Sciatic Nerve Ligation.

Curr Issues Mol Biol 47(6)

Authors: Ai Takahashi, Hiroko Miyagishi, Komugi Tsuruta, Hiroshi Nango, Dai Hirose, Yuri Aono, Minoru Tanigawa, Katsushi Nishimura, Minoru Saito, Takayuki Kawato, Tadashi Saigusa, Yasuhiro Kosuge

Affiliations

  • Laboratory of Pharmacology, School of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi-shi 274-8555, Chiba, Japan.
  • Laboratory of Medical Microbiology, School of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi-shi 274-8555, Chiba, Japan.
  • Department of Pharmacology, School of Dentistry at Matsudo, Nihon University, 2-870-1 Sakaechonishi, Matsudo-shi 271-8587, Chiba, Japan.
  • Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University, Building No. 2, 1-5-1 Kanda Surugadai, Chiyoda, Tokyo 101-0062, Japan.
  • Department of Science and Manufacturing Technology, Junior College, Nihon University, 7-24-1 Narashinodai, Funabashi-shi 274-8501, Chiba, Japan.
  • Department of Biosciences, College of Humanities and Sciences, Nihon University, 3-25-40 Sakurajosui, Setagaya-ku, Tokyo 156-8550, Japan.
  • Department of Oral Health Sciences, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-8310, Japan.

Abstract

Neuropathic pain, characterized by chronic allodynia, remains difficult to manage with current pharmacotherapies. Microglial activation plays a pivotal role in the development and maintenance of neuropathic pain and represents a promising therapeutic target. We previously demonstrated that Miyako Bidens pilosa extract powder (MBP), derived from Miyako Island, Okinawa, suppresses glial activation in a mouse model of amyotrophic lateral sclerosis. In this study, we investigated the analgesic potential of MBP in a mouse model of neuropathic pain. Neuropathic pain was induced in male ICR mice by partial sciatic nerve ligation (PSNL). Mice were orally administered MBP (2 g/kg) or vehicle daily. Mechanical allodynia was assessed using von Frey filaments. On postoperative day 7, MBP-treated mice exhibited significantly reduced allodynia compared to vehicle-treated mice. MBP also attenuated thermal hyperalgesia on postoperative day 7. Lumbar spinal cords (L5) were subjected to immunohistochemical analysis for ionized calcium-binding adaptor molecule 1 (Iba1), a microglial marker. MBP significantly decreased the number of Iba1-positive microglia in the ipsilateral dorsal horn. These results suggest that MBP alleviates neuropathic pain, at least in part, by suppressing microglial activation in the spinal cord. MBP may represent a novel plant-derived therapeutic candidate for treating neuropathic pain.

MeSH

Microglia, Spinal Cord, Pain, Ligation, Sciatic Nerve, Therapeutics, Pharmaceutical Vehicles, Neuralgia, Spine