Journal Article Epublish
PubMed: 40699858 DOI: 10.3390/cimb47060459
A Theoretical and Practical Analysis of Membrane Protein Genes Altered in Neutrophils in Parkinson's Disease.
Curr Issues Mol Biol 47(6)
Authors: Araliz López Pintor, Miriam Nolasco López, José Daniel Lozada-Ramírez, Martín Alejandro Serrano-Meneses, Alicia Ortega Aguilar, Dante Oropeza Canto, César Flores-de Los Ángeles, Victor Hugo Anaya-Muñoz, Aura Matilde Jiménez-Garduño
Affiliations
- Department of Chemical and Biological Sciences, Universidad de las Américas Puebla, Cholula 72810, Mexico.
- Biochemistry Department, Medical Faculty, Universidad Nacional Autónoma de México, Mexico City 04510, Mexico.
- Neurosciences Clinic, Hospital Ángeles Puebla, Puebla 72190, Mexico.
- Molecular Diagnostics Laboratory, Integral Health Center, Universidad Popular Autónoma del estado de Puebla, Puebla 72410, Mexico.
- Escuela Nacional de Estudios Superiores, Unidad Morelia, Universidad Nacional Autónoma de México, Morelia 58190, Mexico.
- Health Sciences Department, Universidad de las Américas Puebla, Cholula 72810, Mexico.
Abstract
Parkinson's disease (PD) is a major health concern, with no accurate or early diagnostic test available for most patients. Chronic inflammation is a recognized contributor to PD pathogenesis; thus, membrane proteins of inflammatory cells such as neutrophils present an accessible target for detecting early molecular changes. In this study, we conducted a theoretical analysis using the GSE99039 database to identify differentially expressed genes (DEGs) in leukocytes from PD patients. From this, we selected nine top candidates for digital polymerase chain reaction (dPCR) analysis in isolated neutrophils from nine PD patients and nine matched controls. Our results revealed significant upregulation of ORAI3 and CLCN2. Unexpectedly, both ACTB (β-actin) and SNCA (alpha-synuclein) were also upregulated in neutrophils. Notably, this study provides the first evidence of CLCN2 expression in neutrophils and demonstrates the significant upregulation of four genes via dPCR. These genes may serve as potential biomarkers for future research on PD detection.
MeSH
Up-Regulation, Genes, Parkinson Disease, Neutrophils, Membrane Proteins, Membranes