Journal Article Epublish
PubMed: 40730482 DOI: 10.4014/jmb.2504.04002
Ethyl Acetate Fraction from Eucommia ulmoides Ameliorates Particulate Matter (PM) 2.5-Induced Intestinal Damage by Restoring Barrier Integrity and Regulating Inflammatory Responses.
J Microbiol Biotechnol 35:e2504002
Authors: Min Ji Kim, Jong Min Kim, Hyo Lim Lee, Ho Jin Heo
Affiliations
- Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea. World Institute of Kimchi, Gwangju 61755, Republic of Korea.
- Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea. Korea Food Research Institute, Wanju 55365, Republic of Korea.
- Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea.
Abstract
This study investigated the protective effect of ethyl acetate fraction from Eucommia ulmoides leaf (EFEL) against intestinal dysfunction induced by chronic exposure to particulate matter (PM) 2.5 in BALB/c mice. EFEL treatment suppressed reactive oxygen species (ROS) production and cellular death from PM 2.5-induced HT29 cells. EFEL supplementation ameliorated PM 2.5-induced intestinal damage by regulating antioxidant biomarkers, including reduced glutathione and superoxide dismutase, malondialdehyde levels, and myeloperoxidase activity. EFEL modulated the gut microbiota composition by increasing beneficial bacteria, such as Lactobacillus and Alistipes, and reducing the abundance of pathogenic bacteria, such as Helicobacter and Clostridia UCG-014, thus contributing to the restoration of the intestinal microenvironment. Furthermore, EFEL regulated the expression of tight junction proteins and inflammatory biomarkers in intestinal tissue. These findings suggest that EFEL may serve as a promising functional food material with the potential to alleviate PM 2.5-induced intestinal barrier dysfunction by regulating oxidative stress, inflammation, and microbiota homeostasis.
MeSH
Tight Junctions, Acetates, Microbiology, Biomarkers, Bacteria, Particulate Matter, Microbiota