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Publications

Journal Article Ahead of print

PubMed: 40784287 DOI: 10.1016/j.psj.2025.105664

Synergistic role of miRNA-1811 and miRNA-302 in enhancing direct reprogramming efficiency of chicken embryo fibroblasts to induced pluripotent stem cells.

Poult Sci 104(11):105664

Authors: Zhenkai Dai, Liqin Liao, Sheng Chen, Zhengzhong Xiao, Xinheng Zhang, Qingmei Xie

Affiliations

  • State Key Laboratory of Swine and Poultry Breeding Industry & Guangdong Laboratory for Lingnan Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China; Henry Fok School of Biology and Agriculture, Shaoguan University, Shaoguan 512005, China; Guangdong Provincial Key Lab of AgroAnimal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China; Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou 510642, PR China; Zhongshan Innovation Center of South China Agricultural University, Zhongshan 528400, PR China.
  • State Key Laboratory of Swine and Poultry Breeding Industry & Guangdong Laboratory for Lingnan Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China; Guangdong Provincial Key Lab of AgroAnimal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China; Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou 510642, PR China; Zhongshan Innovation Center of South China Agricultural University, Zhongshan 528400, PR China.
  • Henry Fok School of Biology and Agriculture, Shaoguan University, Shaoguan 512005, China.
  • State Key Laboratory of Swine and Poultry Breeding Industry & Guangdong Laboratory for Lingnan Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China; Guangdong Provincial Key Lab of AgroAnimal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China; Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou 510642, PR China; Zhongshan Innovation Center of South China Agricultural University, Zhongshan 528400, PR China.

Abstract

The generation of induced pluripotent stem cells (iPSCs) from somatic cells holds immense promise for regenerative medicine and disease modeling. However, the reprogramming efficiency in avian species, such as chickens, has been notably lower than in mammalian models. This study investigates the potential of microRNAs (miRNAs) to enhance the reprogramming of chicken embryo fibroblasts (CEFs) into iPSCs. We report the identification and utilization of gga-miRNA-302s and the novel gga-miRNA-1811, which were found to be co-expressed during early chicken embryonic development. The combination of these miRNAs with key pluripotency genes significantly improved the reprogramming efficiency of CEFs into iPSCs, resulting in cells with robust pluripotency markers and differentiation potential. Our findings demonstrate that co-expression of gga-miRNA-1811 and gga-miRNA-302s plays a critical role in promoting the mesenchymal-to-epithelial transition, a crucial step in the reprogramming process. These results not only advance the field of avian iPSC generation but also offer a valuable strategy for improving reprogramming efficiency across species, with implications for stem cell research and translational medicine.

MeSH

Cells, Microscopy, Electron, Scanning Transmission, Poultry, Pluripotent Stem Cells, Micrornas, Stem Cells, Induced Pluripotent Stem Cells, Fibroblasts